State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Int J Mol Sci. 2023 May 9;24(10):8461. doi: 10.3390/ijms24108461.
Bunge (Danshen) is a traditional Chinese herb with significant medicinal value. The yield and quality of Danshen are greatly affected by climatic conditions, in particular high temperatures. Heat shock factors (Hsfs) play important regulatory roles in plant response to heat and other environmental stresses. However, little is currently known about the role played by the gene family in . Here, we identified 35 genes and classified them into three major groups: SmHsfA (n = 22), SmHsfB (n = 11), and SmHsfC (n = 2) using phylogenetic analysis. The gene structure and protein motifs were relatively conserved within subgroups but diverged among the different groups. The expansion of the gene family was mainly driven by whole-genome/segmental and dispersed gene duplications. The expression profile of in four distinct organs revealed its members (23/35) are predominantly expressed in the root. The expression of a large number of was regulated by drought, ultraviolet, heat and exogenous hormones. Notably, the and genes in SmHsfB2 were the most responsive to heat and are conserved between dicots and monocots. Finally, heterologous expression analysis showed that and enhance thermotolerance in yeast. Our results provide a solid foundation for further functional investigation of in Danshen plants as a response to abiotic stresses.
丹参是一种具有重要药用价值的传统中药。丹参的产量和质量受气候条件影响很大，尤其是高温。热休克因子（Hsf）在植物对热和其他环境胁迫的响应中起着重要的调节作用。然而，目前对于该基因家族在丹参中的作用知之甚少。在这里，我们通过系统发育分析鉴定了 35 个基因，并将它们分为三大类：SmHsfA（n = 22）、SmHsfB（n = 11）和 SmHsfC（n = 2）。亚组内的基因结构和蛋白基序相对保守，但不同组之间存在差异。基因家族的扩张主要是由全基因组/片段和分散基因复制驱动的。在四个不同器官中的表达谱显示其成员（23/35）主要在根中表达。大量的表达受到干旱、紫外线、热和外源激素的调节。值得注意的是，SmHsfB2 中的 和 基因对热最敏感，并且在双子叶植物和单子叶植物之间是保守的。最后，异源表达分析表明 和 增强了酵母的耐热性。我们的研究结果为进一步研究丹参植物作为应对非生物胁迫的响应中的 基因功能提供了坚实的基础。
