抗真菌处理的宿主巨噬细胞对……的影响（原文不完整，翻译可能不准确，需结合完整原文理解）

Effect of Antifungal-Treated Host Macrophages on .

作者信息

Li Hong-Bin, Li Na, Wen Shu-Ran, Qiang Ming-Yue, Yang Zheng-Hui, Dong Tian-Xiang, Li Yu-Ye, Kuang Yi-Qun

机构信息

Department of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China.

NHC Key Laboratory of Drug Addiction Medicine, First Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming 650032, Yunnan, China.

出版信息

Can J Infect Dis Med Microbiol. 2021 Feb 18;2021:8838444. doi: 10.1155/2021/8838444. eCollection 2021.

DOI:10.1155/2021/8838444

PMID:33680221

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7906804/

Abstract

OBJECTIVE

() causes infections associated with severe sepsis and high mortality. This study describes the effects of micafungin (MCF), itraconazole (ICZ), and amphotericin B (AmB) on the function of macrophages during infection.

METHODS

RAW264.1 macrophages were treated with MCF, ICZ, or AmB and then challenged with . Cytokines from infected macrophage supernatants and the levels of superoxide dismutase (SOD) in macrophages were measured at different time points after phagocytosis.

RESULTS

The activity of SOD was significantly increased in RAW264.1 cells that phagocytized and reached a peak level at 6 hours ( < 0.05). ICZ and AmB did not affect the SOD activity in cells that phagocytized versus that in untreated macrophage. stimulated macrophages to secrete cytokines. Neither ICZ nor AmB affected the secretion of interleukin-6 (IL-6), interleukin-8 (IL-8), or tumor necrosis factor- (TNF-) by -infected macrophages. However, MCF downregulated the secretion of TNF- by infected macrophages and reduced the SOD activity of compared with those in untreated controls.

CONCLUSION

Echinocandins may increase their antifungal efficacy by altering the innate immune response of macrophages and attenuating antioxidants of this organism.

摘要

目的

（某因素）导致与严重脓毒症相关的感染及高死亡率。本研究描述了米卡芬净（MCF）、伊曲康唑（ICZ）和两性霉素B（AmB）在（某感染）期间对巨噬细胞功能的影响。

方法

用MCF、ICZ或AmB处理RAW264.1巨噬细胞，然后用（某病原体）进行攻击。在吞噬作用后的不同时间点，测量感染巨噬细胞上清液中的细胞因子以及巨噬细胞中超氧化物歧化酶（SOD）的水平。

结果

吞噬（某病原体）的RAW264.1细胞中SOD的活性显著增加，并在6小时达到峰值水平（P<0.05）。与未处理的巨噬细胞相比，ICZ和AmB对吞噬（某病原体）的细胞中的SOD活性没有影响。（某病原体）刺激巨噬细胞分泌细胞因子。ICZ和AmB均未影响受（某病原体）感染的巨噬细胞分泌白细胞介素-6（IL-6）、白细胞介素-8（IL-8）或肿瘤坏死因子-α（TNF-α）。然而，与未处理的对照组相比，MCF下调了受感染巨噬细胞中TNF-α的分泌，并降低了（某病原体）的SOD活性。