pZL8a：一种从猪粪便中分离出来有待进一步研究的潜在益生菌。
pZL8a: a potential probiotic isolated from pig feces for further research.
作者信息
Jia Dan, Wang Ya, Wang Jiahui, Liu Junlong, Li Hehai, Liu Aihong, Wang Jinming, Guan Guiquan, Luo Jianxun, Yin Hong, Li Youquan
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Yanchangbu, Lanzhou, 730046 Gansu China.
College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050 China.
出版信息
3 Biotech. 2021 Mar;11(3):132. doi: 10.1007/s13205-021-02681-3. Epub 2021 Feb 19.
This study aimed to screen a potential anti-diarrheal probiotic for pigs to meet the growing demand for antibiotic alternatives in livestock. Six intestinal pathogens, (O157: H7) ATCC 43888, ATCC 6538, ATCC 19115, ATCC 14028, ATCC 9207, and ZSY2 were employed as indicator bacteria. Our result showed that pZL8a isolated from pig feces had extensive and higher antibacterial activity against indicator pathogens among 9 tested strains. In addition, valuable attributes of pZL8a such as great tolerance of low pH (3.0) and bile salts (0.3%), high-level adhesion to Caco-2 cells, and similar susceptibility to the reference strain GG (LGG) were observed. Compared with control, pZL8a supplement significantly improved the level of immunoglobulin G (IgG), immunoglobulin M (IgM), and interleukin-2 (IL-2) in mouse serum. Therefore, pZL8a was proposed as a potential probiotic for further research and hope to reduce or replace the application of antibiotics in animal production.
本研究旨在筛选一种潜在的猪用抗腹泻益生菌，以满足畜牧业对抗生素替代品日益增长的需求。使用六种肠道病原体，即（O157：H7）ATCC 43888、ATCC 6538、ATCC 19115、ATCC 14028、ATCC 9207和ZSY2作为指示菌。我们的结果表明，从猪粪便中分离出的pZL8a对9株受试菌株中的指示病原体具有广泛且较高的抗菌活性。此外，还观察到pZL8a的一些有价值的特性，如对低pH（3.0）和胆盐（0.3%）具有很强的耐受性、对Caco-2细胞有高水平的粘附性，以及对参考菌株GG（LGG）有相似的敏感性。与对照组相比，补充pZL8a显著提高了小鼠血清中免疫球蛋白G（IgG）、免疫球蛋白M（IgM）和白细胞介素-2（IL-2）的水平。因此，pZL8a被提议作为一种潜在的益生菌进行进一步研究，有望减少或替代动物生产中抗生素的应用。
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