Huang Dan, Han Huayi, Guo Chen, Lin Xiao, Chen Die, Yang Shu, Yang Qianfan, Li Feng
Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, China.
Nanoscale. 2021 Mar 21;13(11):5706-5713. doi: 10.1039/d0nr09148k. Epub 2021 Mar 8.
Living organisms use interconnected chemical reaction networks (CRNs) to exchange information with the surrounding environment and respond to diverse external stimuli. Inspired by nature, numerous artificial CRNs with a complex information processing function have been recently introduced, with DNA as one of the most attractive engineering materials. Although much progress has been made in DNA-based CRNs in terms of controllable reaction dynamics and molecular computation, the effective integration of signal translation with information processing in a single CRN remains to be difficult. In this work, we introduced a stimuli-responsive DNA reaction network capable of integrated information translation and processing in a stepwise manner. This network is designed to integrate sensing, translation, and decision-making operations by independent modules, in which various logic units capable of performing different functions were realized, including information identification (YES and OR gates), integration (AND and AND-AND gates), integration-filtration (AND-AND-NOT gate), comparison (Comparator), and map-to-map analysis (Feynman gate). Benefitting from the modular and programmable design, continuous and parallel processing operations are also possible. With the innovative functions, we show that the DNA network is a highly useful addition to the current DNA-based CRNs by offering a bottom-up strategy to design devices capable of cascaded information processing with high efficiency.
生物利用相互连接的化学反应网络（CRNs）与周围环境交换信息，并对各种外部刺激做出反应。受自然启发，最近引入了许多具有复杂信息处理功能的人工CRNs，其中DNA是最具吸引力的工程材料之一。尽管基于DNA的CRNs在可控反应动力学和分子计算方面取得了很大进展，但在单个CRN中将信号转换与信息处理有效整合仍然很困难。在这项工作中，我们引入了一种刺激响应性DNA反应网络，能够以逐步方式进行信息的整合转换和处理。该网络旨在通过独立模块整合传感、转换和决策操作，其中实现了各种能够执行不同功能的逻辑单元，包括信息识别（是门和或门）、整合（与门和与与门）、整合过滤（与与非门）、比较（比较器）和映射到映射分析（费曼门）。受益于模块化和可编程设计，连续和并行处理操作也是可能的。凭借这些创新功能，我们表明DNA网络通过提供一种自下而上的策略来设计能够高效进行级联信息处理的设备，是当前基于DNA的CRNs中非常有用的补充。
