Department of Pharmacognosy, College of Pharmacy, Jiamusi University, Jiamusi, 154007, China.
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnosis, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
Glycoconj J. 2021 Apr;38(2):251-259. doi: 10.1007/s10719-021-09982-y. Epub 2021 Mar 9.
Coreopsis tinctoria Nutt. (C.tinctoria) is an annual herb of the Compositae family with many health benefits, such as clearing heat, antioxidant and anticancer activity. In this paper, two polysaccharides were isolated from C.tinctoria, named CTAP-1 and CTAP-2, respectively. Structure of CTAP-1and CTAP-2 were elucidated by high-performance gel permeation chromatography, chemical derivative analyses, GC-MS and NMR techniques. Results reveal that they both CTAP-1 and CTAP-2 consisted of predominant amounts of galacturonic acid residues along with small amounts of arabinose, rhamnose and galactose.Both them contain homogalacturonan and rhammnogalcturan I regions in different ratio, suggesting their pectin-type features. The proliferation activities of CTAP-1 and CTAP-2 on RAW264.7 cells in vitro were detected. Results show both them have the significant proliferation effect on RAW264.7 cells when the concentration from 40 to 200 µg/mL. Given their structural characteristics and proliferation activities, the pectins are expected to be potential natural immune modulators, which need further study.
金鸡菊（Coreopsis tinctoria Nutt.）是菊科一年生草本植物，具有多种保健功效，如清热、抗氧化和抗癌活性。本文从金鸡菊中分离得到两种多糖，分别命名为 CTAP-1 和 CTAP-2。通过高效凝胶渗透色谱、化学衍生分析、GC-MS 和 NMR 技术对 CTAP-1 和 CTAP-2 的结构进行了阐明。结果表明，它们均由大量半乳糖醛酸残基和少量阿拉伯糖、鼠李糖和半乳糖组成。两者均含有不同比例的同质半乳糖醛酸和鼠李半乳糖醛酸 I 区，表明它们具有果胶型特征。体外检测了 CTAP-1 和 CTAP-2 对 RAW264.7 细胞的增殖活性。结果表明，当浓度为 40-200μg/mL 时，两者对 RAW264.7 细胞均有显著的增殖作用。鉴于它们的结构特征和增殖活性，这些果胶有望成为潜在的天然免疫调节剂，需要进一步研究。
