冠状病毒 RNA 基因组中的新型负义基因。

Novel Negative Sense Genes in the RNA Genome of Coronaviruses.

机构信息

Russian-German Academy of Medico-Social and Biotechnological Sciences, Moscow, Russia.

Ivanovsky Institute of Virology, Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia.

出版信息

Dokl Biochem Biophys. 2021 May;496(1):27-31. doi: 10.1134/S1607672921010130. Epub 2021 Mar 10.

DOI:10.1134/S1607672921010130

PMID:33689070

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7944473/

Abstract

The coronavirus family consists of lipid-containing envelope viruses that have a single-stranded RNA genome that encodes 25-30 proteins in different viruses by the mechanism of positive-polarity strategy. In addition, extended open reading trnslation frames (ORFs, genes) located in a negative-sense orientation were found in the genomes of coronaviruses. The size of negative-sense genes varies in the range of 150-450 nt, which corresponds to polypeptides encoded by negative-polarity genes (negative gene proteins, NGP) with m. m. 5-30 × 10 kDa. Coronaviruses show marked differences from virus to virus in the number of negative genes detected. These negative-sense genes in the coronavirus genome allow this family to be considered as viruses developing an ambisense genome strategy.

摘要

冠状病毒科包含有脂质包膜的病毒，其单链 RNA 基因组通过正链极性策略在不同病毒中编码 25-30 种蛋白质。此外，在冠状病毒基因组中还发现了位于负链方向的扩展开放阅读框（ORF，基因）。负链基因的大小在 150-450nt 范围内变化，这对应于由负链基因编码的多肽（负链基因蛋白，NGP），分子量为 5-30×10kDa。冠状病毒在检测到的负链基因数量上与病毒之间存在明显差异。冠状病毒基因组中的这些负链基因使该科病毒被认为是采用双义基因组策略的病毒。