Takano Tomomi, Watanabe Haruna, Doki Tomoyoshi, Kusuhara Hajime
School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
Health and Environment Research Institute, Yokkaichi, Mie 512-1211, Japan.
J Vet Med Sci. 2021 May 9;83(5):805-808. doi: 10.1292/jvms.20-0703. Epub 2021 Mar 11.
Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.
猫诺如病毒（FNoVs）是猫潜在的临床病原体。为了开展FNoV感染的流行病学研究，有必要开发一种简单有效的病毒检测方法。我们研究了一种用于医学实践中检测人类诺如病毒的商用人类诺如病毒定量逆转录聚合酶链反应（RT-PCR）试剂盒是否可用于FNoV检测。该试剂盒能够在实验样本和现场样本中检测出FNoV基因，而不受基因群（GIV和GVI）的影响。基于上述发现，使用人类诺如病毒检测方法来检测FNoVs是可行的。通过将这些方法应用于FNoVs的流行病学调查，可能会阐明FNoV感染与猫肠胃炎之间的关系。
