Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.
Methods Mol Biol. 2021;2265:385-406. doi: 10.1007/978-1-0716-1205-7_28.
Metastatic melanoma is one of the most aggressive types of cancers, diffused worldwide and with a significant percentage of lethality. The employment of animal models to test therapeutic strategies against melanoma growth and metastatic spread is of key relevance for cancer biologists. In this regard, the count of metastatic foci in murine lung tissue is one of the recognized methods to monitor macrometastases of melanoma. Here, we illustrate a clonogenic assay method to detect with high sensitivity the presence of single melanoma cells (micrometastases) at the pulmonary level when metastatic foci are still not detectable in the tissue. This method allows for high precision detection and quantification of melanoma metastatic spread to the lung at early stages.
转移性黑色素瘤是最具侵袭性的癌症之一，在全球范围内广泛存在，且致死率相当高。动物模型的应用对于测试针对黑色素瘤生长和转移扩散的治疗策略至关重要。在这方面，检测鼠肺组织中转移灶的数量是监测黑色素瘤大转移的公认方法之一。在这里，我们描述了一种克隆形成分析方法，该方法可以在组织中仍无法检测到转移灶时，以高灵敏度检测到肺水平单个黑色素瘤细胞（微转移灶）的存在。该方法可在早期高度精确地检测和定量黑色素瘤向肺部的转移扩散。
