Wu Di, Liu Shunjie, Zhou Jing, Chen Runze, Wang Yifan, Feng Zhe, Lin Hui, Qian Jun, Tang Ben Zhong, Cai Xiujun
Department of General Surgery, Sir Run-Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China.
Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Division of Life Science and State Key Laboratory of Molecular Neuroscience, and Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.
ACS Nano. 2021 Mar 23;15(3):5011-5022. doi: 10.1021/acsnano.0c09981. Epub 2021 Mar 11.
Iatrogenic extrahepatic bile duct injury remains a dreaded complication while performing cholecystectomy. Although X-ray based cholangiography could reduce the incidence of biliary tract injuries, the deficiencies including radiation damage and expertise dependence hamper its further clinical application. The effective strategy for intraoperative cholangiography is still urgently required. Herein, a fluorescence-based imaging approach for cholangiography in the near-infrared IIb window (1500-1700 nm) using TT3-CB, a bright aggregation-induced emission luminogen with large π-conjugated planar unit, is reported. In phantom studies, TT3-CB nanoparticles exhibit high near-infrared IIb emission and show better image clarity at varying penetrating depths. When intrabiliary injected into the gallbladder or the common bile duct of the rabbit, TT3-CB nanoparticles enable the real-time imaging of the biliary structure with deep penetrating capability and high signal-to-background ratio. Moreover, the tiny iatrogenic biliary injuries and the gallstones in established disease models could be precisely diagnosed by TT3-CB nanoparticle assisted near-infrared IIb imaging. In summary, we reported a feasible application for aggregation-induced emission dots as biliary contrast agent and realized high-quality cholangiography in the near-infrared IIb window with precise diagnostic ability and nonradioactive damage, which could possibly be applied for intraoperative diagnosis.
医源性肝外胆管损伤仍然是胆囊切除术过程中令人恐惧的并发症。尽管基于X射线的胆管造影可以降低胆道损伤的发生率，但其包括辐射损伤和对专业技术的依赖等缺陷阻碍了其进一步的临床应用。目前仍迫切需要术中胆管造影的有效策略。在此，我们报道了一种基于荧光的成像方法，用于在近红外IIb窗口（1500 - 1700纳米）进行胆管造影，该方法使用TT3-CB，一种具有大π共轭平面单元的明亮聚集诱导发光剂。在模型研究中，TT3-CB纳米颗粒表现出高近红外IIb发射，并在不同穿透深度下显示出更好的图像清晰度。当经胆管注入兔胆囊或胆总管时，TT3-CB纳米颗粒能够对胆管结构进行实时成像，具有深穿透能力和高信噪比。此外，在已建立的疾病模型中，微小的医源性胆管损伤和胆结石可以通过TT3-CB纳米颗粒辅助的近红外IIb成像精确诊断。总之，我们报道了聚集诱导发光点作为胆道造影剂的可行应用，并在近红外IIb窗口实现了具有精确诊断能力和无放射性损伤的高质量胆管造影，这可能适用于术中诊断。
