Zeng Xiaodong, Liao Yuqin, Qiao Xue, Liang Ke, Luo Qiusi, Deng Mingbo, Liu Yishen, Zhang Weijing, Hong Xuechuan, Xiao Yuling
State Key Laboratory of Virology, Department of Cardiology, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Shenzhen Institute of Wuhan University, Shenzhen 518057, China.
Acta Pharm Sin B. 2023 Nov;13(11):4578-4590. doi: 10.1016/j.apsb.2023.07.005. Epub 2023 Jul 7.
Biliary atresia is a rare infant disease that predisposes patients to liver transplantation and death if not treated in time. However, early diagnosis is challenging because the clinical manifestations and laboratory tests of biliary atresia overlap with other cholestatic diseases. Therefore, it is very important to develop a simple, safe and reliable method for the early diagnosis of biliary atresia. Herein, a novel NIR-II fluorescence probe, HZL2, with high quantum yield, excellent biocompatibility, low cytotoxicity and rapid excretion through the liver and gallbladder was developed based on the oil/water partition coefficient and permeability. A simple fecal sample after injection of HZL2 can be used to efficiently identify the success of the mouse model of biliary atresia for the first time, allowing for an early diagnosis of the disease. This study not only developed a simple and safe method for the early diagnosis of biliary atresia with great potential in clinical translation but also provides a research tool for the development of pathogenesis and therapeutic medicines for biliary atresia.
胆道闭锁是一种罕见的婴儿疾病，如果不及时治疗，患者易发生肝移植和死亡。然而，早期诊断具有挑战性，因为胆道闭锁的临床表现和实验室检查与其他胆汁淤积性疾病重叠。因此，开发一种简单、安全、可靠的胆道闭锁早期诊断方法非常重要。在此，基于油/水分配系数和通透性，开发了一种新型近红外二区荧光探针HZL2，其具有高量子产率、优异的生物相容性、低细胞毒性以及通过肝脏和胆囊快速排泄的特性。首次注射HZL2后，一份简单的粪便样本可用于高效鉴定胆道闭锁小鼠模型是否成功，从而实现对该疾病的早期诊断。本研究不仅开发了一种简单安全的胆道闭锁早期诊断方法，在临床转化方面具有巨大潜力，还为胆道闭锁发病机制和治疗药物的研发提供了一种研究工具。
