Department of Laboratory Medicine, Clinical Chemistry, Faculty of Medicine and Health, Örebro University Hospital, Södra Grevrosengatan 1, 703 62, Örebro, Sweden.
School of Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
BMC Cardiovasc Disord. 2021 Mar 11;21(1):131. doi: 10.1186/s12872-021-01914-w.
In healthy, young adults we analyzed a panel of cardiovascular disease related proteins in plasma and compared them with the vascular health of the subjects. The aim was to identify proteins with a relationship to the early atherosclerotic process in healthy individuals.
We employed the proximity extension assay from OLINK proteomics to analyze 92 cardiovascular disease (CVD) related proteins on 833 subjects (men and women, ages 18-26). The women were further divided into an estrogen-using group and non-users. Protein expression was analyzed using principal component analysis (PCA). The following vascular examinations were performed: Pulse-wave velocity (PWV), augmentation index (AIX), carotid-intima media thickness (cIMT).
Three principal components were obtained using PCA to analyze the protein expression. None of the obtained principal components correlated significantly with AIX or cIMT. One of the components, explaining 6% of the total variance of the data, was significantly correlated with PWV. Upon examination of the proteins with the highest factor loadings on this component independently in a multivariable model, adjusting for established CVD risk biomarkers, insulin-like growth factor-binding protein 1 (IGFBP-1) and insulin-like growth factor-binding protein 2 (IGFBP-2) were found to independently, negatively correlate with PWV. Among the established risk factors included in the multivariable model, age was significantly and adversely correlated with all vascular measurements.
In this population of healthy, young adults, groups of CVD related proteins correlate with PWV, but not AIX or cIMT. This group of proteins, of which IGFBP-1 and IGFBP-2 were independently, negatively correlated in a multivariable model with PWV, could have benificial effects on vascular stiffness. The robust association between age and PWV, AIX and cIMT provide insight into the impact of aging on the vasculature, which is detectable even in a population of young, healthy, non-smoking individuals of ages spanning only 8 years.
在健康的年轻成年人中，我们分析了一组与心血管疾病相关的蛋白质在血浆中的表达，并将其与受试者的血管健康状况进行了比较。目的是确定与健康个体早期动脉粥样硬化过程相关的蛋白质。
我们使用 OLINK 蛋白质组学的邻近延伸分析技术，在 833 名受试者（男女，年龄 18-26 岁）中分析了 92 种与心血管疾病（CVD）相关的蛋白质。女性进一步分为雌激素使用者和非使用者组。使用主成分分析（PCA）分析蛋白质表达。进行了以下血管检查：脉搏波速度（PWV）、增强指数（AIX）、颈动脉内膜中层厚度（cIMT）。
使用 PCA 分析蛋白质表达，得到了三个主成分。没有一个获得的主成分与 AIX 或 cIMT 有显著相关性。其中一个成分，解释了数据总方差的 6%，与 PWV 显著相关。在多变量模型中，独立检查该成分上具有最高因子负荷的蛋白质，调整已建立的 CVD 风险生物标志物后，发现胰岛素样生长因子结合蛋白 1（IGFBP-1）和胰岛素样生长因子结合蛋白 2（IGFBP-2）与 PWV 独立负相关。在多变量模型中包含的已建立的危险因素中，年龄与所有血管测量值呈显著负相关。
在这一健康的年轻成年人人群中，一组与 CVD 相关的蛋白质与 PWV 相关，但与 AIX 或 cIMT 不相关。在多变量模型中，IGFBP-1 和 IGFBP-2 与 PWV 独立负相关，这组蛋白质可能对血管僵硬有有益的影响。年龄与 PWV、AIX 和 cIMT 之间的强相关性提供了对血管老化影响的深入了解，即使在一个仅 8 岁跨度的年轻、健康、不吸烟个体的人群中也能检测到这种影响。
