Pella Ricardo, Suárez-Cunza Silvia, Orihuela Patricia, Escudero Francisco, Pérez Ygor, García Mario, Zorrilla Ingrid, Berrío Paola, Romero Sergio
Centro de Fertilidad y Reproducción Asistida CEFRA, Lima, Peru.
Instituto de Bioquímica y Nutrición, Universidad Nacional Mayor de San Marcos, Lima, Peru.
JBRA Assist Reprod. 2020 Jul 14;24(3):296-301. doi: 10.5935/1518-0557.20200012.
To evaluate the follicular fluid oxidative balance of infertile patients of advanced-maternal-age and the correlation between oxidative imbalance in the follicular fluid and the embryological outcomes.
Follicular fluid (FF) from infertile patients of advanced-maternal-age undergoing ART treatments were collected and frozen in cryovials at -86°C until further use, and analyzed at the Biochemistry and Nutrition Institute of San Marcos University. As controls, we used FF from oocyte donors. The FF was then assayed for oxidative balance by ABTS, FRAP and TBARS assays. In order to establish the correlation between oxidative balance and embryo quality, we correlated the number of usable blastocysts (freshly transferred or frozen blastocysts) with the results from ABTS, FRAP and TBARS.
Follicular fluid from patients of Advanced-maternal-age (AMA group) significantly differed from the values found in the control group; the ABTS value was higher and the FRAP value was lower, in comparison to the FF from oocyte donors (control group). The lipid peroxidation was not different between those two groups. Furthermore, there was no significant correlation among the results of the assays, or when correlated with the proportion of usable blastocysts.
Ovarian oxidative balance seems to be critical for oocyte quality in advanced-maternal-age patients; however, we still need more studies on oxidative stress indicators, on a larger set of patients.
评估高龄不孕患者卵泡液的氧化平衡，以及卵泡液氧化失衡与胚胎学结局之间的相关性。
收集接受辅助生殖技术治疗的高龄不孕患者的卵泡液，置于冻存管中，在-86°C下冷冻保存备用，并在圣马科斯大学的生物化学与营养研究所进行分析。作为对照，我们使用了卵母细胞捐赠者的卵泡液。然后通过ABTS、FRAP和TBARS测定法对卵泡液的氧化平衡进行检测。为了确定氧化平衡与胚胎质量之间的相关性，我们将可用囊胚（新鲜移植或冷冻囊胚）的数量与ABTS、FRAP和TBARS的检测结果进行关联分析。
高龄患者（高龄组）的卵泡液与对照组的值有显著差异；与卵母细胞捐赠者的卵泡液（对照组）相比，ABTS值较高，FRAP值较低。两组之间的脂质过氧化没有差异。此外，检测结果之间或与可用囊胚比例之间均无显著相关性。
卵巢氧化平衡似乎对高龄患者的卵母细胞质量至关重要；然而，我们仍需要对更多患者进行关于氧化应激指标的进一步研究。
