Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, United States.
Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, United States.
Front Immunol. 2021 Feb 23;12:616650. doi: 10.3389/fimmu.2021.616650. eCollection 2021.
We have previously shown that obesity is associated with increased secretion of IgG antibodies with anti-self-reactivity. In this paper, we confirm and extend our previous findings. We show that the plasma of individuals with obesity is enriched in autoimmune antibodies whose levels are positively associated with blood frequencies of the subset of Double Negative (DN) B cells, which is the most pro-inflammatory B cell subset. We also show that DN B cells, significantly increased in the blood of obese versus lean individuals, are characterized by higher expression of immune activation markers and of the transcription factor T-bet, both associated with autoimmunity. The removal of DN B cells from the peripheral B cell pool significantly decreases secretion of anti-self IgG antibodies. These results altogether confirm the crucial role of DN B cells in the secretion of anti-self IgG antibodies in individuals with obesity.
我们之前已经表明，肥胖与具有抗自身反应性的 IgG 抗体的分泌增加有关。在本文中，我们证实并扩展了我们之前的发现。我们表明，肥胖个体的血浆富含自身免疫抗体，其水平与双阴性（DN）B 细胞亚群的血液频率呈正相关，DN B 细胞亚群是最具炎症性的 B 细胞亚群。我们还表明，与瘦个体相比，肥胖个体血液中显著增加的 DN B 细胞的特征是免疫激活标志物和转录因子 T-bet 的表达更高，这两者都与自身免疫有关。从外周 B 细胞池中去除 DN B 细胞可显著减少抗自身 IgG 抗体的分泌。这些结果共同证实了 DN B 细胞在肥胖个体中分泌抗自身 IgG 抗体中的关键作用。
