Zhu Sidong, Xue Zehao, Huang Yizhe, Chen Xiunuan, Ren Na, Chen Ting, Chen Yong, Yang Jifang, Chen Jigang
College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China.
Institute of Respiratory and Disease, Shenzhen People's Hospital, Shenzhen 518020, PR China.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004757. Epub 2021 Mar 12.
A Gram-stain-negative bacterium, designated strain 40Bstr401, was isolated from a sediment sample collected from the western Pacific Ocean. Analysis of its 16S rRNA gene sequence revealed that strain 40Bstr401 belongs to the genus and is closely related to type strains Ar-22 (98.2 %), 105 (98.2 %) and BB-My12 (97.4 %). The average nucleotide identity values for 40Bstr401 with Ar-22 and 105 are 79.3 % and 78.8 %, respectively. The DNA-DNA hybridization values between strain 40Bstr401 and Ar-22 and 105 are 26.7 and 26.6 %, respectively. The major isoprenoid quinone of 40Bstr401 is MK-6, and iso-C 3-OH and iso-C are the dominant cellular fatty acids. The major polar lipids are phosphatidylethanolamine, four unidentified amino lipids and two unidentified lipids. The G+C content of the genomic DNA is 42.9 mol%. Its phylogenetic distinctiveness and chemotaxonomic differences, together with the phenotypic properties observed in this study, indicate that strain 40Bstr401 can be differentiated from closely related species. Therefore, we propose strain 40Bstr401 represents a novel species in the genus , for which the name sp. nov. is suggested. The type strain is 40Bstr401 (=MCCC 1K04568=KCTC 82139).
从西太平洋采集的沉积物样本中分离出一株革兰氏阴性菌，命名为40Bstr401菌株。对其16S rRNA基因序列分析表明，40Bstr401菌株属于该属，与模式菌株Ar-22（98.2%）、105（98.2%）和BB-My12（97.4%）密切相关。40Bstr401与Ar-22和105的平均核苷酸同一性值分别为79.3%和78.8%。40Bstr401菌株与Ar-22和105之间的DNA-DNA杂交值分别为26.7%和26.6%。40Bstr401的主要类异戊二烯醌为MK-6，异-C3-OH和异-C为主要细胞脂肪酸。主要极性脂为磷脂酰乙醇胺、四种未鉴定的氨基脂和两种未鉴定的脂。基因组DNA的G+C含量为42.9mol%。其系统发育独特性和化学分类学差异，以及本研究中观察到的表型特征，表明40Bstr401菌株可与密切相关的物种区分开来。因此，我们建议40Bstr401菌株代表该属中的一个新物种，建议命名为sp. nov.。模式菌株为40Bstr401（=MCCC 1K04568=KCTC 82139）。
