Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Jul;63(Pt 7):2672-2677. doi: 10.1099/ijs.0.047647-0. Epub 2013 Jan 4.
A novel Gram-stain-negative, heterotrophic, moderate halophilic and strictly aerobic bacterium, strain 105(T), was isolated from a tidal flat of Taean in Korea. Cells were catalase- and oxidase-positive long rods that showed gliding motility. Optimum temperature, pH and salinity for the growth of strain 105(T) were observed at 30-37 °C, at pH 7.0-7.5, and in the presence of 2-4 % (w/v) NaCl, respectively. The major cellular fatty acids were iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH. Phosphatidylethanolamine and five unidentified lipids were identified as the major polar lipids. The genomic DNA G+C content of strain 105(T) was 42.4 mol% and MK-6 was detected as the predominant isoprenoid quinone. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 105(T) formed a phyletic lineage with members of the genus Muricauda. Strain 105(T) was most closely related to Muricauda aquimarina SW-63(T) (97.6 %), Muricauda beolgyonensis BB-My12(T) (97.5 %), Muricauda lutimaris SMK-108(T) (97.5 %), Muricauda ruestringensis B1(T) (97.3 %), Muricauda flavescens CL-SS4(T) (97.2 %) and Muricauda olearia (96.2 %). The DNA-DNA relatedness values of strain 105(T) with M. aquimarina JCM 11811(T), M. beolgyonensis KCTC 23501(T), M. lutimaris KCTC 22173(T), M. ruestringensis DSM 13258(T) and M. flavescens JCM 11812(T) were 17.2 ± 6.0, 8.7 ± 2.2, 3.7 ± 0.5, 11.0 ± 1.9 and 7.1 ± 1.3 %, respectively. On the basis of phenotypic and molecular features, strain 105(T) represents a novel species of the genus Muricauda, for which the name Muricauda taeanensis sp. nov. is proposed. The type strain is 105(T) ( = KACC 16195(T) = JCM 17757(T)).
一株新的革兰氏阴性、异养、中度嗜盐和严格需氧细菌，菌株 105(T)，从韩国泰安的潮汐滩中分离得到。细胞呈过氧化氢酶和氧化酶阳性的长杆状，具有滑行运动能力。菌株 105(T)的最适生长温度、pH 值和盐度分别为 30-37°C、pH7.0-7.5 和 2-4%(w/v)NaCl。主要的细胞脂肪酸为 iso-C15:1G、iso-C15:0 和 iso-C17:0 3-OH。鉴定出磷脂酰乙醇胺和五种未鉴定的脂质为主要极性脂质。菌株 105(T)的基因组 DNA G+C 含量为 42.4mol%，检测到 MK-6 作为主要的异戊烯醌。基于 16S rRNA 基因序列的系统发育分析表明，菌株 105(T)与 Muricauda 属的成员形成了一个分支。菌株 105(T)与 Muricauda aquimarina SW-63(T)(97.6%)、Muricauda beolgyonensis BB-My12(T)(97.5%)、Muricauda lutimaris SMK-108(T)(97.5%)、Muricauda ruestringensis B1(T)(97.3%)、Muricauda flavescens CL-SS4(T)(97.2%)和 Muricauda olearia(96.2%)最为密切相关。菌株 105(T)与 Muricauda aquimarina JCM 11811(T)、Muricauda beolgyonensis KCTC 23501(T)、Muricauda lutimaris KCTC 22173(T)、Muricauda ruestringensis DSM 13258(T)和 Muricauda flavescens JCM 11812(T)的 DNA-DNA 同源性值分别为 17.2±6.0%、8.7±2.2%、3.7±0.5%、11.0±1.9%和 7.1±1.3%。基于表型和分子特征，菌株 105(T)代表 Muricauda 属的一个新种，因此提议将其命名为 Muricauda taeanensis sp. nov。模式菌株为 105(T)(=KACC 16195(T)=JCM 17757(T))。
