Phyllobacterium pellucidum sp. nov., isolated from soil.

A bacterial strain, BT25, was isolated from soil in Korea. The bacterial cells were Gram-negative and rod-shaped. Phylogenetic analysis using 16S rRNA gene sequences showed that the BT25 strain was related to the genus Phyllobacterium. BT25 was 96.6 and 96.5% similar to Phyllobacterium brassicacearum STM 196 and Phyllobacterium myrsinacearum DSM 5892, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between BT25 and the two closest phylogenetic neighbors were calculated to be 78.5 and 77.7, 21.1 and 21.2%, respectively. The major cellular fatty acids were summed feature 8 (C ω7c/C ω6c) (29.3%), cyclo-C ω8c (27.5%), and C (16.5%). The BT25 strain had menaquinone Q-10 as the predominant quinone, as well as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and phosphatidylcholine as the major polar lipids. Based on the phenotypic, phylogenetic, and chemotaxonomic data, the BT25 strain was classified as a novel Phyllobacterium species. The name Phyllobacterium pellucidum sp. nov. was proposed. The type strain is BT25 (= KCTC 62765 = NBRC 114381).