Masters M
Mol Gen Genet. 1977 Oct 20;155(2):197-202. doi: 10.1007/BF00393160.
The frequencies with which the generalized transducing phage P1 transduced 26 selected markers on the E. coli. chromosome were measured. The frequencies were found to vary relative to argH+ = 1 from a maximum of 6.8 near the origin of replication to a minimum of 0.23 for a marker not far from the terminus. The low frequencies obtained for some markers were shown not to result from poor expression under the selective conditions employed. When plotted as a function of marker position on the chromosome the frequencies were found to exhibit a series of peaks and troughs which correspond to those in gene density noted by Bachmann et al. (1976). The possible relationship of these results to the structure of the E. coli chromosome and to the mechanism of generalized transduction are discussed.
测定了广义转导噬菌体P1转导大肠杆菌染色体上26个选定标记的频率。发现这些频率相对于argH+ = 1有所变化，从复制起点附近的最大值6.8到离终点不远的一个标记的最小值0.23。结果表明，某些标记获得的低频并非由于所用选择条件下表达不佳所致。当将这些频率作为染色体上标记位置的函数作图时，发现它们呈现出一系列的峰和谷，这与巴赫曼等人（1976年）指出的基因密度中的峰和谷相对应。讨论了这些结果与大肠杆菌染色体结构以及广义转导机制的可能关系。
