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耳鼻喉科中无工作通道的软性内镜的基于紫外光的再处理:一种有效的方法吗?

UV light-based reprocessing of flexible endoscopes without working channel in Oto-Rhino-Laryngology: an effective method?

机构信息

Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-Universität Marburg, Baldingerstrasse, 35043, Marburg, Germany.

Department of Medical Microbiology and Hygiene, University Hospital Marburg, Philipps-Universität Marburg, Marburg, Germany.

出版信息

Eur Arch Otorhinolaryngol. 2021 Oct;278(10):4075-4080. doi: 10.1007/s00405-021-06737-1. Epub 2021 Mar 13.

Abstract

BACKGROUND

Reprocessing of flexible endoscopes (FEs) is often expensive, time consuming, and becomes increasingly complex, due to rising demands of hygiene. After beneficial results in reprocessing of rigid endoscopes using Impelux™ UV-C light technology, we tested the same method for reprocessing of FEs without working channel.

MATERIALS AND METHODS

Testing was performed on FEs without working channel after routine clinical use (transnasal flexible endoscopy). Disinfection consisted of mechanical precleaning and 60 s exposure to Impelux™ UV-C light technology. Bacterial contamination was tested on 50 FEs before and after disinfection. Further 50 FEs regarding protein residuals. The absolute effectiveness of the D60 was tested on 50 test bodies (RAMS) with a standardized contamination of 10 colony-forming units (CFU) of Enterococcus faecium.

RESULTS

The FEs were contaminated with a high average value of 916.7 CFU (± 1057 CFU) after clinical usage. After reprocessing, an average contamination of 2.8 CFU (± 1.6) on 14% (n = 7) of the FEs was detected consisting of non-pathogenic species, the remaining FE were sterile. After reprocessing, all FEs were protein-free (< 1 μg). The artificially contaminated test bodies showed no remaining bacterial contamination after disinfection, resulting in an average absolute germ reduction of about 10 CFU.

CONCLUSION

Impelux™ UV-C light technology efficiently reduces bacterial contamination of FEs and might be useful in daily practice.

摘要

背景

由于卫生要求不断提高,软性内镜(FE)的再处理通常既昂贵又耗时,而且变得越来越复杂。在 Impelux™ UV-C 光技术成功应用于硬式内镜的再处理后,我们测试了相同的方法来处理无工作通道的 FE。

材料与方法

对常规临床使用后的无工作通道 FE(经鼻柔性内镜)进行测试。消毒包括机械预清洁和 60 秒暴露于 Impelux™ UV-C 光技术。在消毒前后对 50 个 FE 进行细菌污染测试。对另外 50 个 FE 进行蛋白质残留测试。用 50 个测试体(RAMS)对 D60 的绝对有效性进行测试,标准化污染为 10 个粪肠球菌的菌落形成单位(CFU)。

结果

FE 在临床使用后平均污染程度为 916.7 CFU(±1057 CFU)。再处理后,检测到 14%(n=7)的 FE 平均污染程度为 2.8 CFU(±1.6),包含非致病性物种,其余 FE 无菌。再处理后,所有 FE 均无蛋白质残留(<1μg)。人工污染的测试体在消毒后没有残留细菌污染,平均绝对除菌率约为 10 CFU。

结论

Impelux™ UV-C 光技术可有效降低 FE 的细菌污染,可能在日常实践中有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47c2/8382609/c8d0c8db2657/405_2021_6737_Fig1_HTML.jpg

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