Department of Otolaryngology, Head and Neck Surgery, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany.
Department of Medical Microbiology and Hygiene, University Hospital Marburg, Philipps-University Marburg, 35043 Marburg, Germany.
Int J Environ Res Public Health. 2022 Mar 10;19(6):3246. doi: 10.3390/ijerph19063246.
Background: The reprocessing of medical devices has become more complex due to increasing hygiene requirements. Previous studies showed satisfactory bactericidal disinfection effects of UV-C light in rigid and flexible endoscopes. Especially in the context of the current COVID-19 pandemic, virucidal properties are of high importance. In the present study, the virucidal efficacy of UV-C light surface disinfection was analyzed. Methods: MS-2 bacteriophages were applied to the test samples and irradiated by UV-C light using the UV Smart D25 device; unirradiated test samples were used as controls. A dilution series of the samples was mixed with 1 × 108 Escherichia coli and assayed. Results: 8.6 × 1012 pfu could be harvested from the unprocessed test samples. In the control group without UV-C exposure, a remaining contamination of 1.2 × 1012 pfu was detected, resulting in a procedural baseline reduction rate with a LOG10 reduction factor of 0.72. The LOG10 reduction factor was found to be 3.0 after 25 s of UV-C light exposure. After 50 and 75 s of UV-C radiation LOG10 reduction factors 4.2 and 5.9, respectively, were found, with all reductions being statistically significantly different to baseline. Conclusions: The tested UV system seems to provide a significant virucidal effect after a relatively short irradiation time.
由于卫生要求的提高，医疗器械的再处理变得更加复杂。先前的研究表明，UV-C 光对刚性和柔性内窥镜具有令人满意的杀菌消毒效果。特别是在当前 COVID-19 大流行的背景下，病毒杀灭特性尤为重要。在本研究中，分析了 UV-C 光表面消毒的病毒杀灭效果。
将 MS-2 噬菌体应用于测试样品，并使用 UV Smart D25 设备对其进行 UV-C 光照射；未受照射的测试样品作为对照。对样品进行稀释系列混合 1×108 个大肠杆菌进行检测。
未经处理的测试样品中可收获 8.6×1012 pfu。在未暴露于 UV-C 的对照组中，检测到 1.2×1012 pfu 的残留污染，导致程序基线减少率的 LOG10 减少因子为 0.72。暴露于 25 s 的 UV-C 光后，LOG10 减少因子为 3.0。暴露于 50 和 75 s 的 UV-C 辐射后，LOG10 减少因子分别为 4.2 和 5.9，所有减少均与基线有统计学显著差异。
经过相对较短的照射时间，测试的 UV 系统似乎提供了显著的病毒杀灭效果。
