Institute of Bioproduct Development, Universiti Technologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, Malaysia.
Institute of Bioproduct Development, Universiti Technologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, Malaysia; Department of Bioprocess and Polymer Engineering, Faculty of Chemical and Energy Engineering, Universiti Technologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, Malaysia.
Int J Biol Macromol. 2024 Sep;276(Pt 1):133856. doi: 10.1016/j.ijbiomac.2024.133856. Epub 2024 Jul 14.
Biomolecules, specifically proteins, polysaccharides, and secondary metabolites are potential lead compounds due to their remarkable pharmacological properties. However, the complex molecular structure of the biomolecules makes their separation processes of great challenges. The conventional downstream processes require multistep protocols that are less efficient, high solvent consumption, expensive, time-consuming, and laborious. Hence, aqueous two-phase system (ATPS) is a reliable technique for the extraction and purification of biomolecules from a complex mixture. ATPS is an environmentally friendly, simple, cost effective, and easily scalable process. It requires a short processing time to separate biomolecules of industrial values simultaneously in a single process. Modifications have also been performed by introducing deep eutectic solvents, ionic liquids, carbohydrates, amino acids or copolymers to enhance the process efficiency with an increased yield, purity and bioactivity of recovered biomolecules. This review attempts to review the recent developed ATPSs and their efficiency to extract, isolate, and purify biomolecules such as proteins, polysaccharides, secondary metabolites and other biological substances. The review provides insights into the feasibility and reliability of ATPS for biomolecule recovery.
生物分子，特别是蛋白质、多糖和次生代谢产物，由于其显著的药理学性质，是潜在的先导化合物。然而，生物分子的复杂分子结构使得它们的分离过程极具挑战性。传统的下游工艺需要多步的方案，这些方案效率较低、溶剂消耗量大、成本高、耗时且费力。因此，双水相体系（ATPS）是从复杂混合物中提取和纯化生物分子的可靠技术。ATPS 是一种环保、简单、经济高效且易于扩展的工艺。它需要很短的处理时间，可同时在单个过程中分离具有工业价值的生物分子。通过引入深共晶溶剂、离子液体、碳水化合物、氨基酸或共聚物，也对其进行了改进，以提高过程效率，增加回收生物分子的产量、纯度和生物活性。本文综述了最近开发的 ATPS 及其从蛋白质、多糖、次生代谢产物和其他生物物质中提取、分离和纯化生物分子的效率。本文综述为 ATPS 用于生物分子回收的可行性和可靠性提供了参考。
