Shan Li-Peng, Zhou Yan, Yan Mao-Cang, Liu Lei, Chen Jiong, Chen Jian-Ping
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo 315832, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Meishan Campus, Ningbo University, Ningbo 315832, China.
Zhejiang Mariculture Research Institute, Wenzhou 325005, China.
Virus Res. 2021 May;297:198387. doi: 10.1016/j.virusres.2021.198387. Epub 2021 Mar 11.
White spot syndrome virus (WSSV), a double-stranded DNA virus that infects crustaceans, is the most serious viral pathogen affecting shrimp farming worldwide. To reduce the economic losses caused by WSSV, we screened a novel coumarin derivative from a small molecule drug library, N-(4-((4-(((2-oxo-2H-chromen-7-yl)oxy)methyl)-1H-1,2,3-triazol-1-yl)sulfonyl)phenyl)acetamide (N2905), to evaluate its anti-WSSV effects in vivo. We determined that compound N2905, up to a concentration of 20 mg/L, significantly decreased the number of WSSV copies in Litopenaeus vannamei post-larvae, with a maximum inhibitory rate of > 90 %, and increased the survival rate of WSSV-infected post-larvae. Pre-treatment and post-treatment assays indicated that N2905 could treat, but not prevent, WSSV infections. When WSSV was preincubated with N2905 for 1-4 h, the incidence of viral infections was significantly reduced and survival time of post-larvae extended to 120 h. A stability study of N2905 provided a reference for its practical use. Considering the antiviral stability of N2905 in culture water within 2 d, continuous N2905 exchange was performed, showing a significant decrease in viral load at 120 h post-infection (hpi) and a 55 % increase in survival of WSSV-infected post-larvae. Overall, our study demonstrated the potential of N2905 as an antiviral agent.
白斑综合征病毒（WSSV）是一种感染甲壳类动物的双链DNA病毒，是影响全球对虾养殖的最严重病毒病原体。为减少WSSV造成的经济损失，我们从小分子药物库中筛选了一种新型香豆素衍生物N-(4-((4-(((2-氧代-2H-色烯-7-基)氧基)甲基)-1H-1,2,3-三唑-1-基)磺酰基)phenyl)乙酰胺（N2905），以评估其在体内的抗WSSV效果。我们确定，浓度高达20 mg/L的化合物N2905可显著降低凡纳滨对虾幼体中WSSV拷贝数，最大抑制率>90%，并提高WSSV感染幼体的存活率。预处理和后处理试验表明，N2905可以治疗但不能预防WSSV感染。当WSSV与N2905预孵育1-4小时时，病毒感染的发生率显著降低，幼体的存活时间延长至120小时。N2905的稳定性研究为其实际应用提供了参考。考虑到N2905在2天内在养殖水中的抗病毒稳定性，进行了连续的N2905更换，结果显示感染后120小时（hpi）病毒载量显著降低，WSSV感染幼体的存活率提高了55%。总体而言，我们的研究证明了N2905作为抗病毒剂的潜力。
