Batur Sebnem, Ayla Sule, Sakul Ayse Arzu, Okur Mehmet Evren, Karadag Ayse Esra, Daylan Benay, Ozdemir Ekrem Musa, Kepil Nuray, Gunal Mehmet Yalcin
Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Department of Pathology, Istanbul, Turkey.
Istanbul Medipol University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey.
Medeni Med J. 2020;35(4):315-323. doi: 10.5222/MMJ.2020.89983. Epub 2020 Dec 25.
In this study, we examined the effects of Polypodium vulgare L. (Polypodiaceae) as a candidate to be used for wound healing scarred area. We investigated the antibacterial, and antioxidant activity of P. Vulgare on both in vivo, and in vitro wound healing using an excisional wound model in mice.
We used 32 Balb-c mice equally divided into four groups: Group 1 control, Group 2 vehicle, Group 3 Polypodium vulgare, and Group 4 Centella asiatica extract (CAE). All treatments were applied topically once in a day. The scar area, percentage wound closure and epithelization time were measured. PDGF, VEGF, and collagen immunohistochemical staining were used for evaluation.
CAE and P. vulgare extract groups were observed to be more effective than the control and vehicle groups in terms of new vascular, epidermal and granulation tissue organization. PDGF, VEGF, and collagen immunohistochemical staining was stronger in the P.vulgare extract and CAE groups compared to the control and vehicle groups. In the P. vulgare and CAE groups, PDGF staining intensity was stronger than the control and vehicle groups, but VEGF and collagen staining in P. vulgare group was not different from the control group.
P. vulgare had an effect on the injured area by regenerating the epidermis and increasing vascularization. P. vulgare extract with known antioxidant, and antimicrobial activities may be helpful as a supportive treatment in wound healing.
在本研究中，我们检测了普通水龙骨（水龙骨科）作为用于伤口愈合瘢痕区域候选物的效果。我们使用小鼠切除伤口模型，研究了普通水龙骨在体内和体外伤口愈合方面的抗菌及抗氧化活性。
我们将32只Balb-c小鼠平均分为四组：第1组为对照组，第2组为赋形剂组，第3组为普通水龙骨组，第4组为积雪草提取物（CAE）组。所有处理均每天局部应用一次。测量瘢痕面积、伤口闭合百分比和上皮化时间。采用血小板衍生生长因子（PDGF）、血管内皮生长因子（VEGF）和胶原蛋白免疫组化染色进行评估。
在新血管、表皮和肉芽组织形成方面，观察到CAE组和普通水龙骨提取物组比对照组和赋形剂组更有效。与对照组和赋形剂组相比，普通水龙骨提取物组和CAE组的PDGF、VEGF和胶原蛋白免疫组化染色更强。在普通水龙骨组和CAE组中，PDGF染色强度比对照组和赋形剂组更强，但普通水龙骨组的VEGF和胶原蛋白染色与对照组无差异。
普通水龙骨通过再生表皮和增加血管生成对损伤区域产生影响。具有已知抗氧化和抗菌活性的普通水龙骨提取物可能有助于作为伤口愈合的辅助治疗。
