Du Gongbo, Ma Runsheng, Li Hongqiang, He Jiao, Feng Kaixiang, Niu Dongpeng, Yin Detao
Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Key Discipline Laboratory of Clinical Medicine Henan, Zhengzhou, China.
Front Oncol. 2021 Feb 26;11:644011. doi: 10.3389/fonc.2021.644011. eCollection 2021.
Circular RNA (circRNA) is a newly discovered non-coding RNA. Recent reports suggest that circRNAs are key regulators of tumorigenesis because of their special structure. In order to investigate the role of hsa_circ_0002111 in papillary thyroid cancer (PTC), we use quantitative real-time polymerase chain reaction (qRT-PCR) to determine the expression pattern of hsa_circ_0002111 in 82 paired PTC and adjacent non-cancerous thyroid tissues. Cell counting kit-8, colony formation, and transwell assays were conducted to assess the effect of hsa_circ_0002111 on PTC cell proliferation, migration, and invasion. We found that the expression of hsa_circ_0002111 was significantly up-regulated in PTC tissues compared with adjacent non-cancerous tissues (P < 0.0001). Expression of hsa_circ_0002111 was also associated with advanced TNM stage and lymph-node metastasis of patients with PTC. The area under the receiver operating characteristic curve was 0.833. Further, cell function assays showed that hsa_circ_0002111 inhibition significantly suppressed the proliferation and invasion abilities of PTC cells . In conclusions, the study findings show that the over-expression of hsa_circ_0002111 promotes PTC, and thus hsa_circ_0002111 may be a potential diagnostic biomarker and therapeutic target for PTC.
环状RNA（circRNA）是一种新发现的非编码RNA。最近的报道表明，由于其特殊结构，circRNAs是肿瘤发生的关键调节因子。为了研究hsa_circ_0002111在甲状腺乳头状癌（PTC）中的作用，我们使用定量实时聚合酶链反应（qRT-PCR）来确定hsa_circ_0002111在82对PTC及相邻非癌甲状腺组织中的表达模式。进行细胞计数试剂盒-8、集落形成和Transwell实验以评估hsa_circ_0002111对PTC细胞增殖、迁移和侵袭的影响。我们发现，与相邻非癌组织相比，hsa_circ_0002111在PTC组织中的表达显著上调（P < 0.0001）。hsa_circ_0002111的表达也与PTC患者的晚期TNM分期和淋巴结转移相关。受试者工作特征曲线下面积为0.833。此外，细胞功能实验表明，hsa_circ_0002111抑制可显著抑制PTC细胞的增殖和侵袭能力。总之，研究结果表明hsa_circ_0002111的过表达促进PTC，因此hsa_circ_0002111可能是PTC的潜在诊断生物标志物和治疗靶点。
