Department of Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, China.
Shenzhen Promegene Technology Co., Ltd, Shenzhen, China.
Front Cell Infect Microbiol. 2021 Feb 25;11:549678. doi: 10.3389/fcimb.2021.549678. eCollection 2021.
Gestational diseases are associated with altered intestinal microbiota in pregnant women. Characterizing the gut microbiota of gestational anemia (GA) may describe a novel role of gut microbial abnormality in GA. In this study, we investigated differences in gut microbiota between GA patients and healthy pregnant women from the first trimester (n = 24 54) and the third trimester (n = 30 56) based on the 16S rRNA gene sequencing method. No statistically significant differences in α-diversity were identified between GA patients and controls in the first trimester of pregnancy, whereas the Shannon index and observed OTUs were significantly lower in GA patients than in healthy controls in the third trimester. Distance-based redundancy analysis revealed striking differences in microbial communities in the third trimester between GA patients and controls. Four genera were significantly different in relative abundance between GA patients and healthy controls, while 12 genera differentiated significantly between GA patients and healthy controls in the third trimester. At the operational taxonomic unit (OTU) level, 17 OTUs and 30 OTUs were identified to be different between GA patients and healthy controls in the first and third trimesters, respectively. Changes in gut microbial composition of GA patients suggest a potential relation with GA, and provide insights into the prediction and intervention of gestational anemia.
妊娠期疾病与孕妇肠道微生物群改变有关。描述妊娠期贫血（GA）的肠道微生物群特征可能描述了肠道微生物异常在 GA 中的新作用。在这项研究中，我们基于 16S rRNA 基因测序方法，调查了 24 名处于妊娠第一 trimester（n = 54）和 30 名处于妊娠第三 trimester（n = 56）的 GA 患者与健康孕妇之间肠道微生物群的差异。在妊娠第一 trimester 时，GA 患者与对照组之间的 α-多样性没有统计学上的显著差异，而在妊娠第三 trimester 时，GA 患者的 Shannon 指数和观察到的 OTUs 明显低于健康对照组。基于距离的冗余分析显示，妊娠第三 trimester 时 GA 患者与对照组之间的微生物群落存在显著差异。四个属的相对丰度在 GA 患者和健康对照组之间存在显著差异，而在妊娠第三 trimester 时，有 12 个属在 GA 患者和健康对照组之间存在显著差异。在操作分类单元（OTU）水平上，在妊娠第一和第三 trimester 时，分别有 17 个和 30 个 OTUs 在 GA 患者和健康对照组之间存在差异。GA 患者肠道微生物组成的变化表明与 GA 有潜在关系，并为预测和干预妊娠期贫血提供了思路。
