Molenaar W M, Dam-Meiring A, Kamps W A, Cornelisse C J
Department of Pathology, University of Groningen, The Netherlands.
Hum Pathol. 1988 May;19(5):573-9. doi: 10.1016/s0046-8177(88)80207-7.
In the current study DNA-ploidy was determined in a group of 11 desmin-positive rhabdomyosarcomas and found to be aneuploid in all of them. In three cases, synchronous metastases could be studied as well. In two of them a hypotetraploid peak was observed not present in the primary tumor. In two other cases residual tumors obtained after chemotherapy appeared to have DNA-profiles similar to those observed in their primary tumors. These findings suggest either a selection of metastasizing clones or tumor progression. No apparent selection of clones responding to therapy could be established. For comparison, a group of other childhood sarcomas (four Ewing's sarcomas, two primitive neuroectodermal tumors, and two epithelioid sarcomas) was also studied. All except one of these cases appeared to have diploid or near-diploid stemlines. These results suggest that DNA-ploidy may be used as an additional criterion in the differential diagnosis of childhood sarcomas.
在当前研究中，对一组11例结蛋白阳性横纹肌肉瘤进行了DNA倍性测定，发现所有病例均为非整倍体。其中3例还对同步转移灶进行了研究。在其中2例中观察到一个次四倍体峰，而原发肿瘤中不存在该峰。在另外2例中，化疗后获得的残留肿瘤的DNA谱与原发肿瘤中观察到的相似。这些发现提示可能存在转移克隆的选择或肿瘤进展。未发现对治疗有反应的克隆有明显选择。作为对照，还对一组其他儿童肉瘤（4例尤因肉瘤、2例原始神经外胚层肿瘤和2例上皮样肉瘤）进行了研究。除1例病例外，所有这些病例似乎都具有二倍体或近二倍体干细胞系。这些结果表明，DNA倍性可作为儿童肉瘤鉴别诊断的一个附加标准。
