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经长读测序改进的 全基因组序列。

Improved Whole-Genome Sequence of Generated by Long-Read Sequencing.

机构信息

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.

Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

出版信息

Mol Plant Microbe Interact. 2021 Jul;34(7):866-869. doi: 10.1094/MPMI-12-20-0356-A. Epub 2021 Aug 24.

Abstract

The soilborne oomycete is the most destructive pathogen of vegetable crops and is responsible for substantial economic losses worldwide. Here, we present an improved genome assembly of generated by Oxford Nanopore long-read sequencing (for de novo assembly) and Illumina short-read sequencing (for polishing). The genome of is 100.5 Mb in length (GC content = 50.8%) and contains 26,069 predicted protein-coding genes. The whole genome of is assembled into 194 scaffolds, 90% of which are larger than 300 kb. The N scaffold length and maximum scaffold length are 1.0 and 4.1 Mb, respectively. The whole-genome sequence of will broaden our knowledge of this pathogen and enhance our understanding of the molecular basis of its pathogenicity, which will facilitate the development of effective management strategies.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

摘要

土壤鞭毛菌是蔬菜作物最具破坏性的病原体,是造成全球重大经济损失的罪魁祸首。在这里,我们展示了一种改良的基因组组装,该组装由牛津纳米孔长读测序(从头组装)和 Illumina 短读测序(用于抛光)生成。 的基因组长度为 100.5Mb(GC 含量= 50.8%),包含 26069 个预测的蛋白质编码基因。 的全基因组组装成 194 个支架,其中 90%大于 300kb。N 支架长度和最大支架长度分别为 1.0 和 4.1Mb。 的全基因组序列将拓宽我们对这种病原体的认识,并增强我们对其致病性分子基础的理解,这将有助于制定有效的管理策略。[公式:见正文]版权所有 © 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。

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