• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

经长读测序改进的 全基因组序列。

Improved Whole-Genome Sequence of Generated by Long-Read Sequencing.

机构信息

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.

Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

出版信息

Mol Plant Microbe Interact. 2021 Jul;34(7):866-869. doi: 10.1094/MPMI-12-20-0356-A. Epub 2021 Aug 24.

DOI:10.1094/MPMI-12-20-0356-A
PMID:33720746
Abstract

The soilborne oomycete is the most destructive pathogen of vegetable crops and is responsible for substantial economic losses worldwide. Here, we present an improved genome assembly of generated by Oxford Nanopore long-read sequencing (for de novo assembly) and Illumina short-read sequencing (for polishing). The genome of is 100.5 Mb in length (GC content = 50.8%) and contains 26,069 predicted protein-coding genes. The whole genome of is assembled into 194 scaffolds, 90% of which are larger than 300 kb. The N scaffold length and maximum scaffold length are 1.0 and 4.1 Mb, respectively. The whole-genome sequence of will broaden our knowledge of this pathogen and enhance our understanding of the molecular basis of its pathogenicity, which will facilitate the development of effective management strategies.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

摘要

土壤鞭毛菌是蔬菜作物最具破坏性的病原体,是造成全球重大经济损失的罪魁祸首。在这里,我们展示了一种改良的基因组组装,该组装由牛津纳米孔长读测序(从头组装)和 Illumina 短读测序(用于抛光)生成。 的基因组长度为 100.5Mb(GC 含量= 50.8%),包含 26069 个预测的蛋白质编码基因。 的全基因组组装成 194 个支架,其中 90%大于 300kb。N 支架长度和最大支架长度分别为 1.0 和 4.1Mb。 的全基因组序列将拓宽我们对这种病原体的认识,并增强我们对其致病性分子基础的理解,这将有助于制定有效的管理策略。[公式:见正文]版权所有 © 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。

相似文献

1
Improved Whole-Genome Sequence of Generated by Long-Read Sequencing.经长读测序改进的 全基因组序列。
Mol Plant Microbe Interact. 2021 Jul;34(7):866-869. doi: 10.1094/MPMI-12-20-0356-A. Epub 2021 Aug 24.
2
Draft Assembly of from Long-Read Sequencing Uncovers Complexity.从头测序组装揭示复杂性。
Mol Plant Microbe Interact. 2019 Dec;32(12):1559-1563. doi: 10.1094/MPMI-04-19-0103-TA. Epub 2019 Oct 16.
3
Genome Sequence Resource of from China Using Nanopore Sequencing Technology.中国利用纳米孔测序技术获得的 基因组序列资源。
Plant Dis. 2021 Dec;105(12):4141-4145. doi: 10.1094/PDIS-11-20-2327-A. Epub 2021 Dec 3.
4
Draft Genome Resource of f. sp. , the Infectious Agent of Pepper Wilt.f. sp. 的基因组草案资源, 胡椒枯萎病的传染性病原体。
Mol Plant Microbe Interact. 2021 Jun;34(6):715-717. doi: 10.1094/MPMI-12-20-0355-A. Epub 2021 Jul 21.
5
Quantitative Genetic Analysis of Interactions in the Pepper- Pathosystem.定量遗传分析胡椒-病原体系统中的相互作用。
Mol Plant Microbe Interact. 2022 Nov;35(11):1018-1033. doi: 10.1094/MPMI-12-21-0307-R. Epub 2022 Nov 1.
6
Genome Sequence Data of , an Oomycete Pathogen Causing Litchi Downy Blight.荔枝霜疫霉基因组序列数据,一种引起荔枝霜霉病的卵菌病原体。
Mol Plant Microbe Interact. 2021 Jun;34(6):707-710. doi: 10.1094/MPMI-11-20-0303-A. Epub 2021 Jul 7.
7
sORF-Encoded Polypeptide SEP1 Is a Novel Virulence Factor of Pathogens.sORF 编码多肽 SEP1 是病原体的一种新型毒力因子。
Mol Plant Microbe Interact. 2021 Feb;34(2):157-167. doi: 10.1094/MPMI-06-20-0160-R. Epub 2021 Jan 11.
8
Improved Whole-Genome Sequence of , the Fungal Pathogen Causing Fusarium Head Blight in Rice.改进的水稻镰孢穗枯病真菌病原体全基因组序列。
Mol Plant Microbe Interact. 2022 Jan;35(1):85-89. doi: 10.1094/MPMI-07-21-0182-A. Epub 2021 Dec 17.
9
RNAi-Based Gene Silencing of RXLR Effectors Protects Plants Against the Oomycete Pathogen .基于 RNAi 的 RXLR 效应子基因沉默可保护植物免受卵菌病原体侵害。
Mol Plant Microbe Interact. 2022 Jun;35(6):440-449. doi: 10.1094/MPMI-12-21-0295-R. Epub 2022 Apr 26.
10
Genome Sequence Resource of YC2-1, a Fungal Pathogen Causing Phomopsis Stem Blight in Soybean.YC2-1,一种引起大豆茎枯病的真菌病原体的基因组序列资源。
Mol Plant Microbe Interact. 2021 Jul;34(7):842-844. doi: 10.1094/MPMI-12-20-0340-A. Epub 2021 Aug 15.

引用本文的文献

1
Long-read sequencing to detect full-length protein-protein interactions.用于检测全长蛋白质-蛋白质相互作用的长读长测序。
Sci Rep. 2025 Jul 17;15(1):25967. doi: 10.1038/s41598-025-08549-3.
2
Genomic and transcriptomic analyses of reveal complex genome architecture, expansion of pathogenicity factors, and host-dependent gene expression profiles.对……的基因组和转录组分析揭示了复杂的基因组结构、致病因子的扩展以及宿主依赖性基因表达谱。 需注意,原文中“of”后面缺少具体所指内容,翻译时补充了“……”来表示这一缺失部分。
Front Microbiol. 2024 Aug 15;15:1341803. doi: 10.3389/fmicb.2024.1341803. eCollection 2024.
3
A conserved oomycete effector RxLR23 triggers plant defense responses by targeting ERD15La to release NbNAC68.
一种保守的卵菌效应物 RxLR23 通过靶向 ERD15La 释放 NbNAC68 来触发植物防御反应。
Nat Commun. 2024 Jul 27;15(1):6336. doi: 10.1038/s41467-024-50782-3.
4
genome assembly for two isolates using long-read Oxford Nanopore Technology sequencing.使用长读长牛津纳米孔技术测序对两个分离株进行基因组组装。
Microbiol Resour Announc. 2023 Dec 14;12(12):e0019623. doi: 10.1128/MRA.00196-23. Epub 2023 Nov 10.
5
Sterol-Sensing Domain (SSD)-Containing Proteins in Sterol Auxotrophic Mediate Sterol Signaling and Play a Role in Asexual Reproduction and Pathogenicity.固醇感应结构域(SSD)蛋白在固醇营养缺陷型中参与固醇信号转导,并在无性生殖和致病性中发挥作用。
Microbiol Spectr. 2023 Feb 14;11(1):e0379722. doi: 10.1128/spectrum.03797-22. Epub 2023 Jan 11.
6
Polymorphic Microsatellite Development, Genetic Diversity, Population Differentiation and Sexual State of Phytophthora capsici on Commercial Peppers in Three Provinces of Southwest China.中国西南三省商业辣椒上辣椒疫霉的多态微卫星开发、遗传多样性、种群分化和有性状态。
Appl Environ Microbiol. 2022 Dec 13;88(23):e0161122. doi: 10.1128/aem.01611-22. Epub 2022 Nov 10.
7
Identification and functional deciphering suggested the regulatory roles of long intergenic ncRNAs (lincRNAs) in increasing grafting pepper resistance to Phytophthora capsici.鉴定和功能解析表明长非编码 RNA(lncRNA)在提高嫁接辣椒对辣椒疫霉抗性方面的调控作用。
BMC Genomics. 2021 Dec 2;22(1):868. doi: 10.1186/s12864-021-08183-z.
8
Genome Analysis of JM01 Provides Insights into Its Pathogenicity Mechanisms.JM01的基因组分析为其致病机制提供了见解。
Plants (Basel). 2021 Aug 6;10(8):1620. doi: 10.3390/plants10081620.
9
Comparative Analysis of Host-Associated Variation in .宿主相关变异的比较分析……(原文不完整,翻译到此处)
Front Microbiol. 2021 Jul 2;12:679936. doi: 10.3389/fmicb.2021.679936. eCollection 2021.