染色体蛋白与DNA的序列特异性相互作用。
Sequence specific interaction of the chromosomal proteins with DNA.
作者信息
Tuan D, Chetsanga C, Doty P M
出版信息
Nucleic Acids Res. 1977 Oct;4(10):3415-39. doi: 10.1093/nar/4.10.3415.
Calf thymus chromatin was partially deproteinized by 0.6-1 M NaCl extraction. From the shape of the temperature derivative plot of its melting curve, the DNA in each chromatin was resolved into regions of exposed DNA, and DNA still complexed with proteins. The partially deproteinized chromatin was capable of directing in vitro RNA synthesis in an amount proportional to the fraction of exposed DNA. The in vitro RNA transcript was hybridized to denatured calf DNA under hybridization conditions where only RNA's transcribed from repetitive DNA sequences were able to form hybrids. From the results it is deduced that the in vitro RNA transcripts of 0.6 M NaC1-extracted chromatin after the removal of HI histones plus one-quater of the non histones may be transcribed from repetitive DNA sequences and those of 1 M NaCl-extracted chromatin contain more transcripts from the unique DNA sequences.
通过0.6 - 1M NaCl提取法对小牛胸腺染色质进行部分脱蛋白处理。根据其熔解曲线的温度导数图的形状，将每个染色质中的DNA解析为暴露的DNA区域以及仍与蛋白质复合的DNA区域。部分脱蛋白的染色质能够指导体外RNA合成，合成量与暴露DNA的比例成正比。在仅由重复DNA序列转录的RNA能够形成杂交体的杂交条件下，将体外RNA转录本与变性的小牛DNA进行杂交。从结果推断，去除H1组蛋白加四分之一的非组蛋白后，0.6M NaCl提取的染色质的体外RNA转录本可能来自重复DNA序列，而1M NaCl提取的染色质的转录本包含更多来自单一DNA序列的转录物。
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