人α和β珠蛋白基因的mRNA相关序列碱基组成的物理化学测定

Physicochemical measurement of the base composition of mRNA-related sequences of the human alpha and beta globin genes.

作者信息

Tuan D, Forget B G

出版信息

Mol Biol Rep. 1980 Oct 31;6(3):179-83. doi: 10.1007/BF00775413.

Abstract

Hybrids formed between human alpha and beta globin cDNA and total human cellular DNA have been studied by thermal denaturation and cesium chloride density gradient centrifugation. From these studies, the weight average G + C content of human alpha globin cDNA has been determined to be 62% +/- 2% and that of human beta globin cDNA 51% +/- 2%. These values correlate well with the results of G + C content of the human alpha and beta globin cDNAs as determined by direct nucleotide sequence analysis of the cDNAs. Thermal denaturation and cesium chloride density gradient centrifugation of DNA-cDNA hybrids can therefore provide accurate information on the base composition of mRNA related sequences of any single copy gene for which a relatively pure cDNA can be obtained, without the necessity for direct nucleotide sequence analysis.

摘要

通过热变性和氯化铯密度梯度离心法研究了人α和β珠蛋白cDNA与人类细胞总DNA形成的杂交体。通过这些研究，已确定人α珠蛋白cDNA的重量平均G + C含量为62%±2%，人β珠蛋白cDNA的重量平均G + C含量为51%±2%。这些值与通过对cDNA进行直接核苷酸序列分析所确定的人α和β珠蛋白cDNA的G + C含量结果高度相关。因此，DNA-cDNA杂交体的热变性和氯化铯密度梯度离心法可以为任何能获得相对纯cDNA的单拷贝基因的mRNA相关序列的碱基组成提供准确信息，而无需进行直接核苷酸序列分析。