酵母苯丙氨酸转运核糖核酸的脉冲傅里叶变换核磁共振双共振研究：特定的核Overhauser效应及低温弛豫数据的重新解释
Pulsed FT-NMR double resonance studies of yeast tRNAPhe: specific nuclear Overhauser effects and reinterpretation of low temperature relaxation data.
作者信息
Johnston P D, Redfield A G
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3913-27. doi: 10.1093/nar/5.10.3913.
Abstract
Cross-relaxation effects are demonstrated between the imino protons and other protons in yeast tRNAPhe and H2O. A detailed examination has been made of the observed relaxation rate of the proton resonance at 11.8 ppm from DSS as a function of the D2O content in the solvent. This result, as well as the size and number of observed nuclear Overhauser effects, suggests that dipolar magnetization transfer between solvent H2O, amino, imino, and other tRNA protons may dominate the relaxation processes of the imino protons at low temperature. At higher temperatures the observed relaxation rate is dominated by chemical exchange. The selective nuclear Overhauser effects are shown to be an important aid in resonance assignments. By these means we were able to identify tow protons from the wobble base pair GU4 at 11.8 ppm and 10.4 ppm.
摘要
在酵母苯丙氨酸转运核糖核酸（tRNAPhe）和水（H2O）中，亚氨基质子与其他质子之间表现出交叉弛豫效应。已对以DSS为参照在11.8 ppm处质子共振的观测弛豫速率作为溶剂中重水（D2O）含量的函数进行了详细研究。这一结果以及观测到的核Overhauser效应的大小和数量表明，在低温下，溶剂水、氨基、亚氨基和其他转运核糖核酸质子之间的偶极磁化转移可能主导亚氨基质子的弛豫过程。在较高温度下，观测到的弛豫速率由化学交换主导。选择性核Overhauser效应被证明对共振归属有重要帮助。通过这些方法，我们能够在11.8 ppm和10.4 ppm处从摆动碱基对GU4中识别出两个质子。
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