乳糖操纵子DNA亚氨基质子交换动力学与其功能的相关性。
Correlation of lac operator DNA imino proton exchange kinetics with its function.
作者信息
Cheung S, Arndt K, Lu P
出版信息
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3665-9. doi: 10.1073/pnas.81.12.3665.
Abstract
The kinetics for imino hydrogen exchange, at individual base pairs in the DNA sequence corresponding to the lactose operon operator of Escherichia coli, has been examined by NMR saturation recovery measurements as a function of temperature. Three 17-base-pair subsections of the lac operator DNA were chemically synthesized for these studies. The results support our previous observations in the 36-base-pair complete lac operator DNA fragment that has been used in our previous NMR studies. The results indicate faster opening kinetics at a GTG/CAC that is also the site of operator mutations leading to the highest level of constitutive beta-galactosidase synthesis. The GTG/CAC sequence occurs frequently and often symmetrically in prokaryotic and eukaryotic DNA sites where one anticipates specific protein interaction for gene regulation or recombination.
摘要
通过核磁共振饱和恢复测量，研究了与大肠杆菌乳糖操纵子操纵基因对应的DNA序列中各个碱基对处亚氨基氢交换的动力学，该动力学是温度的函数。为这些研究化学合成了lac操纵基因DNA的三个17个碱基对的子片段。结果支持了我们之前在36个碱基对的完整lac操纵基因DNA片段中的观察结果，该片段已用于我们之前的核磁共振研究。结果表明，在GTG/CAC处的开放动力学更快，该位点也是导致组成型β-半乳糖苷酶合成水平最高的操纵基因突变位点。GTG/CAC序列在原核和真核DNA位点中频繁且通常对称出现，在这些位点人们预期会发生特定的蛋白质相互作用以进行基因调控或重组。
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