Department of Veterinary Public Health & Epidemiology, NTR College of Veterinary Science, Gannavaram, Krishna District 521102, Andhra Pradesh, India.
J Food Prot. 2021 Aug 1;84(8):1374-1379. doi: 10.4315/JFP-20-399.
Proteus mirabilis is abundant in soil and water. Although this bacterium is part of the normal human intestinal flora, it can cause serious infections in humans, including complicated urinary tract infections. This pathogen is also commonly associated with multidrug resistance. In the present study, analysis of 1,093 samples from foods of animal origin and animal intestinal samples recovered 232 P. mirabilis isolates identified by PCR assay. Of these 232 isolates, 72 produced β-lactamase (determined by both phenotypic and genotypic methods), with the highest prevalence in poultry cloacal swabs (11.82%) followed by mutton (9.18%), khoa (6.32%), pork (5.63%), pig rectal swabs (5.52%), beef (5.45%), and chicken (5.13%) but none from sheep rectal swabs and bovine rectal swabs. Among β-lactamase genes, blaTEM was the predominant gene detected (59 isolates) followed by blaOXA (11 isolates), blaSHV (5 isolates), blaFOX (5 isolates), blaCIT (4 isolates), blaCTX-M1 and blaCTX-M9 (2 isolates each) and blaCTX-M2, blaDHA, and blaEBC (1 isolate each). None of the isolates carried blaACC, blaMOX, or carbapenemase genes (blaVIM, blaIMP, blaKPC, and blaNDM-1). Dendrogram analysis of enterobacterial repetitive intergenic consensus sequences and repetitive extragenic palindromic sequences obtained with PCR analysis of β-lactamase-producing isolates revealed 63 isolates, but 9 isolates did not yield bands. The analysis revealed that 6.58% of the samples had β-lactamase-producing P. mirabilis isolates that may affect food safety and contaminate the environment. Further genotyping revealed the genetic relationships between isolates of different origin. These findings emphasize the need for careful use of antibiotics to control the spread of β-lactamase-producing bacteria.
奇异变形杆菌广泛存在于土壤和水中。尽管该细菌是人类肠道正常菌群的一部分，但它可引起人类严重感染，包括复杂的尿路感染。这种病原体通常也与多药耐药性有关。在本研究中，通过 PCR 检测分析了 1093 份来自动物源性食品和动物肠道样本，共分离出 232 株奇异变形杆菌。在这 232 株分离株中，有 72 株产生β-内酰胺酶（通过表型和基因型方法确定），其中在禽类泄殖腔拭子中的检出率最高（11.82%），其次是羊肉（9.18%）、 khoa（6.32%）、猪肉（5.63%）、猪直肠拭子（5.52%）、牛肉（5.45%）和鸡肉（5.13%），但在绵羊直肠拭子和牛直肠拭子中均未检出。在β-内酰胺酶基因中，检测到的主要基因是 blaTEM（59 株），其次是 blaOXA（11 株）、blaSHV（5 株）、blaFOX（5 株）、blaCIT（4 株）、blaCTX-M1 和 blaCTX-M9（各 2 株）和 blaCTX-M2、blaDHA 和 blaEBC（各 1 株）。没有分离株携带 blaACC、blaMOX 或碳青霉烯酶基因（blaVIM、blaIMP、blaKPC 和 blaNDM-1）。用 blaTEM 产生菌的 PCR 分析获得的肠杆菌重复基因间共有序列和重复外回文序列的聚类分析显示有 63 株，但有 9 株未产生条带。分析表明，有 6.58%的样品含有可能影响食品安全和污染环境的产β-内酰胺酶奇异变形杆菌分离株。进一步的基因分型显示了不同来源分离株之间的遗传关系。这些发现强调了需要谨慎使用抗生素来控制β-内酰胺酶产生菌的传播。
