利用定向进化的力量来改进基因组编辑系统。
Harnessing the power of directed evolution to improve genome editing systems.
机构信息
Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States.
Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States.
出版信息
Curr Opin Chem Biol. 2021 Oct;64:10-19. doi: 10.1016/j.cbpa.2021.02.004. Epub 2021 Mar 13.
The recent development of genome editing systems, such as zinc-finger nucleases, transcription activator-like effectors, CRISPR-Cas nucleases, and base editors has enabled the unprecedented capability to engineer the genomes of living cells. The ever-increasing demand for genome editors with improved accuracy, activity, and functionality has stimulated significant efforts to further engineer the genome editing systems. Directed evolution represents a promising strategy to improve the existing genome editing systems and enable new editing functions. Here, we review recent representative strategies to harness the power of directed evolution to improve genome editing systems, which have led to state-of-the-art genome editors that have significant implications for diverse applications in both laboratories and clinics.
近年来，基因组编辑系统（如锌指核酸酶、转录激活因子样效应物、CRISPR-Cas 核酸酶和碱基编辑器）的发展使得对活细胞基因组进行工程改造成为可能。人们对具有更高精度、活性和功能的基因组编辑工具的需求不断增加，这极大地促进了对基因组编辑系统的进一步工程化改造。定向进化是一种提高现有基因组编辑系统并实现新编辑功能的很有前途的策略。在这里，我们综述了利用定向进化的力量来改进基因组编辑系统的最新代表性策略，这些策略已经产生了最先进的基因组编辑工具，这些工具对实验室和临床中的各种应用具有重要意义。
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