Petry Fernanda, Dall'Orsoleta Bruna B, Scatolin Mikaela, Morgan Leticia V, Alves Bianca O, Anzollin Gabriela S, Zilli Gabriela A L, Scapinello Jaqueline, Danielli Leticia J, Soares Krissie D, Apel Miriam, Oliveira J Vladimir, Magro Jacir Dal, Müller Liz G
Graduate Program in Environmental Sciences, Community University of Chapecó Region (Unochapecó), Chapecó 89809-900, Brazil.
Health Sciences Area, Community University of Chapecó Region (Unochapecó), Chapecó 89809-900, Brazil.
Evid Based Complement Alternat Med. 2021 Feb 26;2021:6670544. doi: 10.1155/2021/6670544. eCollection 2021.
is a plant species traditionally used in the treatment of diabetes, fever, hypercholesterolemia, obesity, and urinary tract diseases. The anti-inflammatory effects of leaves in mice were already known. Nevertheless, studies on the anti-inflammatory activity of its seeds are still lacking. The aim of this study was to investigate the anti-inflammatory activity and acute toxicity of seed extract, obtained from supercritical CO extraction (SCCO) at 40°C and 250 bar, in mice. GC/MS analysis revealed that -caryophyllene is the major compound present in the SCCO extract. The extract (60 mg/kg, p.o.) significantly reduced the nociceptive behavior in the second phase of the formalin test and prevented the paw oedema induced by carrageenan up to 6 h after carrageenan injection. The extract (0.1-1 g/mL) inhibited neutrophils migration induced by LPS from . This antichemostatic effect was comparable to the effect of indomethacin. Acute administration (2000 mg/kg, p.o.) of SCCO extract caused no mice mortality, demonstrating that the extract is devoid of acute toxicity. These data suggest that seeds present anti-inflammatory activity and represent a source of anti-inflammatory compounds.
是一种传统上用于治疗糖尿病、发热、高胆固醇血症、肥胖症和泌尿系统疾病的植物物种。其叶子在小鼠中的抗炎作用早已为人所知。然而，关于其种子抗炎活性的研究仍然缺乏。本研究的目的是研究从40°C和250巴的超临界CO萃取（SCCO）获得的种子提取物在小鼠中的抗炎活性和急性毒性。气相色谱/质谱分析表明，β-石竹烯是SCCO提取物中的主要化合物。提取物（60mg/kg，口服）在福尔马林试验的第二阶段显著降低了伤害性反应行为，并在注射角叉菜胶后长达6小时预防了角叉菜胶诱导的爪肿胀。提取物（0.1-1g/mL）抑制了脂多糖诱导的中性粒细胞从……迁移。这种抗止血作用与吲哚美辛的作用相当。急性给予（2000mg/kg，口服）SCCO提取物未导致小鼠死亡，表明该提取物无急性毒性。这些数据表明种子具有抗炎活性，是抗炎化合物的一个来源。
