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原核 tRNA 输出在原生动物中：一个充满曲折的旅程，由 tRNA 修饰引导。

Nuclear tRNA export in trypanosomes: a journey full of twists and turns guided by tRNA modifications.

机构信息

Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweiss), Czech Republic.

Faculty of Science, University of South Bohemia, České Budějovice (Budweiss), Czech Republic.

出版信息

Parasitology. 2021 Sep;148(10):1219-1222. doi: 10.1017/S0031182021000482. Epub 2021 Mar 17.

DOI:10.1017/S0031182021000482
PMID:33729118
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8311961/
Abstract

Transfer RNAs play a key role in protein synthesis. Following transcription, tRNAs are extensively processed prior to their departure from the nucleus to become fully functional during translation. This includes removal of 5′ leaders and 3′ trailers by a specific endo- and/or exonuclease, 3′ CCA tail addition, posttranscriptional modifications and in some cases intron removal. In this minireview, the critical factors of nuclear tRNA trafficking are described based on studies in classical models such as yeast and human cell lines. In addition, recent findings and identification of novel regulatory loops of nuclear tRNA trafficking in trypanosomes are discussed with emphasis on tRNA modifications. The comparison between the representatives of opisthokonts and excavates serves here to understand the evolutionary conservation and diversity of nuclear tRNA export mechanisms.

摘要

转移 RNA 在蛋白质合成中发挥着关键作用。在转录后，tRNA 在离开细胞核之前要经过广泛的加工，才能在翻译过程中完全发挥功能。这包括通过特定的内切核酸酶和/或外切核酸酶去除 5' 前导序列和 3' 尾随序列、3' CCA 尾添加、转录后修饰以及在某些情况下切除内含子。在这篇综述中，基于酵母和人类细胞系等经典模型的研究，描述了核 tRNA 运输的关键因素。此外，还讨论了最近在原生动物中发现的核 tRNA 运输的新调控环，并重点讨论了 tRNA 修饰。这里比较后口动物和挖掘动物的代表，旨在了解核 tRNA 输出机制的进化保守性和多样性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cb3/11532560/2738b15bda5f/S0031182021000482_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cb3/11532560/f1bac515add8/S0031182021000482_figAb.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cb3/11532560/2738b15bda5f/S0031182021000482_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cb3/11532560/f1bac515add8/S0031182021000482_figAb.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cb3/11532560/2738b15bda5f/S0031182021000482_fig1.jpg

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引用本文的文献

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Parasitology. 2021 Sep;148(10):1119-1124. doi: 10.1017/S0031182021000998. Epub 2021 Jun 14.

本文引用的文献

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tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei.LCCR4 通过 tRNA 3' 缩短来应对布鲁氏锥虫中的应激反应。
Nucleic Acids Res. 2021 Feb 22;49(3):1647-1661. doi: 10.1093/nar/gkaa1261.
2
A novel assay provides insight into tRNAPhe retrograde nuclear import and re-export in S. cerevisiae.一种新的测定方法为研究酿酒酵母中 tRNAPhe 的逆行核输入和再输出提供了新的视角。
Nucleic Acids Res. 2020 Nov 18;48(20):11577-11588. doi: 10.1093/nar/gkaa879.
3
The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei.
Mex67 和 Mtr2 这两种一般的 mRNA 输出蛋白在布氏锥虫的 tRNA 核输出中发挥不同的作用。
Nucleic Acids Res. 2019 Sep 19;47(16):8620-8631. doi: 10.1093/nar/gkz671.
4
Oxidative Stress Triggers Selective tRNA Retrograde Transport in Human Cells during the Integrated Stress Response.氧化应激在人细胞的综合应激反应中触发选择性 tRNA 逆行运输。
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tRNA Processing and Subcellular Trafficking Proteins Multitask in Pathways for Other RNAs.转运RNA加工与亚细胞运输蛋白在其他RNA的通路中承担多种功能。
Front Genet. 2019 Feb 20;10:96. doi: 10.3389/fgene.2019.00096. eCollection 2019.
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A tRNA half modulates translation as stress response in Trypanosoma brucei.tRNA 半分子作为应激反应调节锥虫中的翻译。
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Dynamics of intracellular stress-induced tRNA trafficking.细胞内应激诱导的 tRNA 运输的动态变化。
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Sharing the load: Mex67-Mtr2 cofunctions with Los1 in primary tRNA nuclear export.分担负荷：Mex67-Mtr2与Los1在tRNA初级核输出过程中协同发挥作用。
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9
tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: Location, location, location.tRNA 在细胞核、细胞质和线粒体表面之间的动态变化：位置、位置、位置。
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