Nguyen Phuong T M, Nguyen Minh T H, Bolhuis Albert
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Viet Nam.
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Viet Nam.
Saudi J Biol Sci. 2021 Mar;28(3):1615-1621. doi: 10.1016/j.sjbs.2020.11.061. Epub 2020 Nov 24.
This study aimed to investigate the antibiofilm activity of alpha-mangostin (AMG) loaded nanoparticles (nanoAMG) against , including the methicillin-resistant strain MRSA252. The results indicated that treatment with 24 μmol/L nanoAMG inhibited the formation of biofilm biomass by 53-62%, compared to 40-44% for free AMG (p < 0.05). At 48 μmol/L, biofilms in all nanoAMG treated samples were nearly fully disrupted for the two tested strains, MRSA252 and the methicillin-sensitive strain NCTC6571. That concentration resulted in killing of biofilm cells. A lower concentration of 12 µmol/L nanoAMG inhibited initial adherence of the two bacterial strains by > 50%. In contrast, activity of nanoAMG was limited on preformed mature biofilms, which at a concentration of 48 µmol/L were reduced only by 27% and 22% for NCTC6571 and MRSA252, respectively. The effects of AMG or nanoAMG on the expression of biofilm-related genes showed some noticeable differences between the two strains. For instance, the expression level of was downregulated in MRSA252 and upregulated in NCTC6571 when those strains were treated with either AMG or nanoAMG. In contrast, the expression of was down regulated in NCTC6571, while it was up-regulated in the MRSA252. The expression of other biofilm-related genes (, and ) was down regulated in both strains. In conclusion, our results suggest that AMG coated nanoparticles had enhanced biological activity as compared to free AMG, indicating that nanoAMG could be a new and promising inhibitor of biofilm formation to tackle , including strains that are resistant to multiple antibiotics.
本研究旨在调查负载α-山竹素（AMG）的纳米颗粒（nanoAMG）对包括耐甲氧西林菌株MRSA252在内的细菌的抗生物膜活性。结果表明，与游离AMG的40 - 44%相比，用24μmol/L nanoAMG处理可使生物膜生物量的形成减少53 - 62%（p < 0.05）。在48μmol/L时，对于两种测试菌株MRSA252和甲氧西林敏感菌株NCTC6571，所有经nanoAMG处理的样品中的生物膜几乎完全被破坏。该浓度导致生物膜细胞死亡。较低浓度的12μmol/L nanoAMG可使两种细菌菌株的初始黏附减少> 50%。相比之下，nanoAMG对预先形成的成熟生物膜的活性有限，在48μmol/L浓度下，NCTC6571和MRSA252的生物膜分别仅减少27%和22%。AMG或nanoAMG对生物膜相关基因表达的影响在两种菌株之间显示出一些明显差异。例如，当用AMG或nanoAMG处理这些菌株时，MRSA252中 的表达水平下调，而在NCTC6571中上调。相比之下，NCTC6571中 的表达下调，而在MRSA252中上调。两种菌株中其他生物膜相关基因（ 、 和 ）的表达均下调。总之，我们的结果表明，与游离AMG相比，AMG包被的纳米颗粒具有增强的生物活性，这表明nanoAMG可能是一种新型且有前景的生物膜形成抑制剂，可用于应对包括对多种抗生素耐药的菌株在内的细菌。
