Hoque Hammadul, Islam Rahatul, Ghosh Srijon, Rahaman Md Mashiur, Jewel Nurnabi Azad, Miah Md Abunasar
Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh.
Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali, Bangladesh.
Heliyon. 2021 Mar 8;7(3):e06396. doi: 10.1016/j.heliyon.2021.e06396. eCollection 2021 Mar.
Being a Positive sense RNA virus the recent reemergence of Chikungunya and Mayaro virus has taken the concern of the leading scientific communities of the world. Though the outbreak of Mayaro virus is limited to Neotropical region only, Chikungunya is already identified in over 60 countries around the world. Besides, the lack of a strong protective treatment, misdiagnosis issue and co-circulation of both the viruses calls for a new strategy which could potentially prevent these infections from spreading. In this study, we therefore, identified the peptide based vaccine candidates e.g. epitopes for B cell and T cell from Chikungunya virus which also showed to be homologous to the Mayaro virus through immuno-informatics and computational approaches. Final epitopes identified from the most antigenic structural polyprotein of both the viruses were 5 for CD8+ T cell Epitopes (KPGDSGRPI, TGTMGHFIL, ALSVVTWNK, KPGRRERMC and GRRERMCMK), 2 epitopes for CD4+ T cell (MCMKIENDCIFEVKH and DRTLLSQQSGNVKIT) and a single epitope for B cell (GGRFTIPTGAGKPGDSGRPI). Analysis of our predicted epitopes for population coverage showed prominent population coverage (92.43%) around the world. Finally, molecular docking simulation of the foreseen T cell epitopes with respondent HLA alleles secured good HLA-epitope interaction. This study was directed towards the discovery of potential antigenic epitopes which can open up a new skyline to design novel vaccines for combating both of the diseases at the same time.
作为一种正链RNA病毒，基孔肯雅病毒和马亚罗病毒近期的再度出现引起了全球主要科学界的关注。虽然马亚罗病毒的爆发仅局限于新热带地区，但基孔肯雅病毒已在全球60多个国家被发现。此外，由于缺乏有效的保护性治疗方法、误诊问题以及两种病毒的共同传播，需要一种新的策略来预防这些感染的传播。因此，在本研究中，我们通过免疫信息学和计算方法，从基孔肯雅病毒中鉴定出基于肽的候选疫苗，例如B细胞和T细胞表位，这些表位也与马亚罗病毒具有同源性。从两种病毒的最具抗原性的结构多聚蛋白中鉴定出的最终表位，CD8 + T细胞表位有5个（KPGDSGRPI、TGTMGHFIL、ALSVVTWNK、KPGRRERMC和GRRERMCMK），CD4 + T细胞表位有2个（MCMKIENDCIFEVKH和DRTLLSQQSGNVKIT），B细胞表位有1个（GGRFTIPTGAGKPGDSGRPI）。对我们预测的表位进行人群覆盖率分析显示，在全球范围内有显著的人群覆盖率（92.43%）。最后，对预测的T细胞表位与相应的HLA等位基因进行分子对接模拟，确保了良好的HLA - 表位相互作用。本研究旨在发现潜在的抗原表位，这可为同时对抗这两种疾病设计新型疫苗开辟新的前景。
