对宿主和注射的致癌细胞的时间过程转录组分析揭示了免疫防御的新方面。
A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects of immune defenses.
机构信息
Sino-French Hoffmann Institute, School of Basic Medical Science, Guangzhou Medical University, 511436 Guangzhou, China;
Insect Models of Innate Immunity (M3I; UPR9022), CNRS, University of Strasbourg, F-67084 Strasbourg, France.
出版信息
Proc Natl Acad Sci U S A. 2021 Mar 23;118(12). doi: 10.1073/pnas.2100825118.
Oncogenic RasV12 cells [A. Simcox et al., 4, e1000142 (2008)] injected into adult males proliferated massively after a lag period of several days, and led to the demise of the flies after 2 to 3 wk. The injection induced an early massive transcriptomic response that, unexpectedly, included more than 100 genes encoding chemoreceptors of various families. The kinetics of induction and the identities of the induced genes differed markedly from the responses generated by injections of microbes. Subsequently, hundreds of genes were up-regulated, attesting to intense catabolic activities in the flies, active tracheogenesis, and cuticulogenesis, as well as stress and inflammation-type responses. At 11 d after the injections, GFP-positive oncogenic cells isolated from the host flies exhibited a markedly different transcriptomic profile from that of the host and distinct from that at the time of their injection, including in particular up-regulated expression of genes typical for cells engaged in the classical antimicrobial response of .
致癌性 RasV12 细胞[A. Simcox 等人，4，e1000142（2008）]注入成年雄性果蝇后，经过几天的潜伏期后大量增殖，并在 2 至 3 周后导致果蝇死亡。注射诱导了一个早期的大规模转录组反应，出人意料的是，该反应包括 100 多个编码各种家族化学感受器的基因。诱导的动力学和诱导基因的特性与由微生物注射产生的反应明显不同。随后，数百个基因被上调，证明果蝇中存在强烈的分解代谢活性、活跃的气管发生和表皮发生，以及应激和炎症类型的反应。在注射后 11 天，从宿主果蝇中分离出的 GFP 阳性致癌细胞表现出与宿主明显不同的转录组谱，也与注射时的转录组谱不同，特别是参与经典抗菌反应的基因表达上调。
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