Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Instituto de Biotecnología Ambiental y Salud, INBIAS-CONICET, Ruta Nacional 36 Km 601 (CP 5800), Río Cuarto, Córdoba, Argentina.
Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Instituto de Investigaciones Agrobiotecnológicas, INIAB-CONICET, Ruta Nacional 36 Km 601 (CP 5800), Río Cuarto, Córdoba, Argentina.
Environ Sci Pollut Res Int. 2021 Aug;28(29):38604-38612. doi: 10.1007/s11356-021-13529-x. Epub 2021 Mar 18.
Vicia villosa Roth is a legume species with a growing application in Argentina as a cover crop (CC), a practice that favors the sustainable development of agricultural systems. However, several areas where the use of this CC provides numerous advantages are affected by high concentrations of arsenic (As). Thus, in the present work we studied hairy vetch ability to cope with arsenate [As(V)], arsenite [As(III)], and the mixture of both along with oxidative stress indexes [chlorophyll content, malondialdehyde (MDA) equivalents] as well as anatomical and histological changes in the root structure. The results obtained suggested a different behavior of hairy vetch depending on its growth stage and on metal(oid) concentration. The roots treated with the contaminant showed less turgidity, thickening of the epidermal and subepidermal parenchymal outer layers, and the presence of dark deposits. The morpho-anatomic parameters (cortex length, vascular cylinder diameter, total diameter, and vascular cylinder area) were altered in plants treated with As(V) and As(V)/As(III) whereas the roots of plants treated with As(III) did not show significant differences respect to the control. Moreover V. villosa could tolerate and remove As from soil, thus the use of this legume species seems an attractive approach to remediate As while protecting contaminated soils.
野豌豆是一种豆科植物，在阿根廷作为覆盖作物（CC）的应用越来越广泛，这种做法有利于农业系统的可持续发展。然而，在一些该 CC 具有诸多优势的地区，砷（As）的浓度却很高。因此，在本工作中，我们研究了野豌豆应对砷酸盐[As(V)]、亚砷酸盐[As(III)]以及两者混合物的能力，以及氧化应激指标（叶绿素含量、丙二醛（MDA）当量），以及根结构的解剖和组织学变化。结果表明，野豌豆的行为因生长阶段和金属（类）浓度的不同而不同。受污染物处理的根显示出较低的膨压、表皮和表皮下外皮层的增厚，以及存在暗沉积物。用 As(V)和 As(V)/As(III)处理的植物的形态解剖学参数（皮层长度、维管束直径、总直径和维管束面积）发生了改变，而用 As(III)处理的根与对照相比没有明显差异。此外，野豌豆可以耐受和从土壤中去除 As，因此，使用这种豆科植物似乎是一种有吸引力的方法，可以在保护受污染土壤的同时修复 As。
