CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R. China; University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
First Hospital of Lanzhou University, Lanzhou 730000, P.R. China.
Talanta. 2017 Apr 1;165:625-631. doi: 10.1016/j.talanta.2017.01.015. Epub 2017 Jan 7.
In view of the important roles of reactive oxygen species (ROS) and reactive sulfur species (RSS) in the complex signal transduction and oxidation pathways, fluorescent probes that are able to display distinct signals to hypochlorous acid and SO derivatives are highly valuable. Herein, a novel dual-functional probe (DFP) as an efficient single fluorescent-molecule which can respond to HClO and HSO with two different sets of fluorescence signals was presented. The DFP displayed desired properties such as high specificity, suitable sensitivity, appreciable water solubility and stability. The sensing mechanism was confirmed by high-resolution mass spectroscopy analysis and H NMR spectrometry analysis. Moreover, as a biocompatible molecule, the DFP has been successfully applied for the detection of HClO in living cells with a dual-channel mode. Therefore, the present work established a novel strategy for monitoring the multiple ROS and RSS species using a single fluorescent probe and the DFP is promising as a molecular tool to investigate the production and dynamics of HClO and HSO in the complex interaction networks of the living system.
鉴于活性氧（ROS）和活性硫（RSS）在复杂的信号转导和氧化途径中发挥着重要作用，能够对次氯酸和 SO 衍生物显示出独特信号的荧光探针具有很高的价值。在此，提出了一种新型双功能探针（DFP），它是一种高效的单荧光分子，能够用两套不同的荧光信号来响应 HClO 和 HSO。DFP 表现出高特异性、适当的灵敏度、可观的水溶性和稳定性等理想特性。通过高分辨率质谱分析和 1H NMR 光谱分析证实了传感机制。此外，作为一种生物相容性分子，DFP 已成功应用于活细胞中 HClO 的双通道检测。因此，本工作建立了一种使用单个荧光探针监测多种 ROS 和 RSS 物种的新策略，DFP 有望成为研究活系统复杂相互作用网络中 HClO 和 HSO 产生和动力学的分子工具。
