Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds vej 14, DK-8000 Aarhus C, Denmark.
Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds vej 14, DK-8000 Aarhus C, Denmark; Department of Bioscience, Aarhus University, Ny Munkegade 114, DK-8000 Aarhus C, Denmark.
Curr Opin Biotechnol. 2015 Jun;33:73-80. doi: 10.1016/j.copbio.2014.12.002. Epub 2014 Dec 18.
Bacterial biofilms endure high concentrations of biocides, and new strategies for biofilm control must therefore replace or complement the use of antibiotics, for example, by targeting the extracellular matrix to cause dispersal or increased antimicrobial susceptibility. Extracellular DNA (eDNA) is a matrix component of most biofilms, and is therefore an attractive target. Enzymatic degradation of eDNA can prevent, disperse, or sensitize biofilm to antimicrobials, but cheaper production is required to realize large-scale application. Replacing mammalian DNase with bacterial nucleases could offer a path to lower production costs. Alternatively, eDNA could be targeted by disrupting its interactions with other matrix components. As new knowledge about eDNA-binding matrix components comes to light, exciting opportunities for targeting the biofilm matrix via eDNA are emerging.
细菌生物膜能承受高浓度的杀菌剂，因此，必须采用新的策略来控制生物膜，以替代或补充抗生素的使用，例如通过靶向细胞外基质来导致分散或增加抗菌药物敏感性。细胞外 DNA（eDNA）是大多数生物膜的基质成分，因此是一个有吸引力的靶点。酶促降解 eDNA 可以阻止、分散或使生物膜对抗微生物药物敏感，但需要更低的生产成本来实现大规模应用。用细菌核酸酶替代哺乳动物 DNA 酶可能是降低生产成本的一种途径。或者，可以通过破坏 eDNA 与其他基质成分的相互作用来靶向 eDNA。随着有关 eDNA 结合基质成分的新知识不断涌现，通过 eDNA 靶向生物膜基质的机会正在出现。
