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见(序列)而后得之:完整单细胞中的高级别基因组折叠。

See(quence) and ye shall find: higher-order genome folding in intact single cells.

机构信息

Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA; Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA; Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; New York Stem Cell Foundation - Robertson Investigator.

出版信息

Mol Cell. 2021 Mar 18;81(6):1130-1132. doi: 10.1016/j.molcel.2021.02.035.

DOI:10.1016/j.molcel.2021.02.035
PMID:33740473
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9295612/
Abstract

Payne et al. (2020) combine in situ imaging and ex situ sequencing via spatially resolved unique molecular barcodes to query higher-order genome folding patterns in intact single nuclei from mouse embryos and human fibroblasts.

摘要

佩恩等人(2020 年)通过空间分辨的独特分子条形码将原位成像和非原位测序相结合,以查询来自小鼠胚胎和人成纤维细胞的完整单个核中的高级别基因组折叠模式。

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本文引用的文献

1
Integrated spatial genomics reveals global architecture of single nuclei.整合空间基因组学揭示了单细胞的全局结构。
Nature. 2021 Feb;590(7845):344-350. doi: 10.1038/s41586-020-03126-2. Epub 2021 Jan 27.
2
In situ genome sequencing resolves DNA sequence and structure in intact biological samples.原位基因组测序可解析完整生物样本中的DNA序列和结构。
Science. 2021 Feb 26;371(6532). doi: 10.1126/science.aay3446. Epub 2020 Dec 31.
3
Genome-Scale Imaging of the 3D Organization and Transcriptional Activity of Chromatin.基因组规模的染色质三维组织和转录活性成像。
Cell. 2020 Sep 17;182(6):1641-1659.e26. doi: 10.1016/j.cell.2020.07.032. Epub 2020 Aug 20.
4
3D mapping and accelerated super-resolution imaging of the human genome using in situ sequencing.利用原位测序对人类基因组进行 3D 图谱绘制和加速超分辨率成像。
Nat Methods. 2020 Aug;17(8):822-832. doi: 10.1038/s41592-020-0890-0. Epub 2020 Jul 27.
5
Visualizing DNA folding and RNA in embryos at single-cell resolution.在单细胞分辨率下可视化胚胎中的 DNA 折叠和 RNA。
Nature. 2019 Apr;568(7750):49-54. doi: 10.1038/s41586-019-1035-4. Epub 2019 Mar 18.
6
Walking along chromosomes with super-resolution imaging, contact maps, and integrative modeling.用超高分辨率成像、接触图谱和整合建模沿着染色体行走。
PLoS Genet. 2018 Dec 26;14(12):e1007872. doi: 10.1371/journal.pgen.1007872. eCollection 2018 Dec.
7
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.超分辨率染色质追踪揭示了单细胞中的域和协同相互作用。
Science. 2018 Oct 26;362(6413). doi: 10.1126/science.aau1783.
8
A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping.一份碱基对分辨率的人类基因组三维图谱揭示了染色质环化的原理。
Cell. 2014 Dec 18;159(7):1665-80. doi: 10.1016/j.cell.2014.11.021. Epub 2014 Dec 11.
9
Architectural protein subclasses shape 3D organization of genomes during lineage commitment.结构蛋白亚类在谱系确定过程中塑造基因组的 3D 结构。
Cell. 2013 Jun 6;153(6):1281-95. doi: 10.1016/j.cell.2013.04.053. Epub 2013 May 23.
10
Topological domains in mammalian genomes identified by analysis of chromatin interactions.哺乳动物基因组中通过分析染色质相互作用而鉴定的拓扑结构域。
Nature. 2012 Apr 11;485(7398):376-80. doi: 10.1038/nature11082.