Ganganboina Akhilesh Babu, Dega Naresh Kumar, Tran Hai Linh, Darmonto Win, Doong Ruey-An
Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Japan.
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 101, Sec. 2, Kuang Fu Road, Hsinchu, 30013, Taiwan.
Biosens Bioelectron. 2021 Jun 1;181:113151. doi: 10.1016/j.bios.2021.113151. Epub 2021 Mar 10.
Glioma is the predominant brain tumor with high death rate. The successful development of biosensor to achieve an efficient detection of glioma cells at low concentration remains a great challenge for the personalized glioma therapy. Herein, an ultrasensitive pulse induced electrochemically impedimetric biosensor for glioma cells detection has been successfully fabricated. The 4-11 nm sulfur-doped graphene quantum dots (S-GQDs) are homogeneously deposited onto gold nanoparticles decorated carbon nanospheres (Au-CNS) by Au-thiol linkage to form S-GQDs@Au-CNS nanocomposite which acts as dual functional probe for enhancing the electrochemical activity as well as conjugating the angiopep-2 (Ang-2) for glioma cell detection. Moreover, the application of an externally electrical pulse at +0.6 V expend the surface of glioma cells to accelerate the attachment of glioma cells onto the Ang-2-conjugated S-GQDs@Au-CNS nanocomposite, resulting in the enhanced sensitivity toward glioma cell detection. An ultrasensitive impedimetric detection of glioma cells with a wide linear range of 100-100,000 cells mL and a limit of detection of 40 cells mL is observed. Moreover, the superior selectivity with long-term stability of the developed biosensor in human serum matrix corroborates the feasibility of using S-GQDs@Au-CNS based nanomaterials as the promising sensing probe for practical application to facilitate the ultrasensitive and highly selective detection of cancer cells.
胶质瘤是死亡率很高的主要脑部肿瘤。成功开发出能在低浓度下有效检测胶质瘤细胞的生物传感器，对于个性化胶质瘤治疗仍然是一个巨大挑战。在此，已成功制备出一种用于检测胶质瘤细胞的超灵敏脉冲诱导电化学阻抗生物传感器。通过金硫键将4 - 11纳米的硫掺杂石墨烯量子点（S - GQDs）均匀沉积在金纳米颗粒修饰的碳纳米球（Au - CNS）上，形成S - GQDs@Au - CNS纳米复合材料，该复合材料作为双功能探针，可增强电化学活性并结合血管活性肠肽 - 2（Ang - 2）用于胶质瘤细胞检测。此外，在 +0.6 V施加外部电脉冲可使胶质瘤细胞表面扩张，加速胶质瘤细胞附着到结合了Ang - 2的S - GQDs@Au - CNS纳米复合材料上，从而提高对胶质瘤细胞检测的灵敏度。观察到对胶质瘤细胞的超灵敏阻抗检测，线性范围宽达100 - 100,000个细胞/毫升，检测限为40个细胞/毫升。此外，所开发的生物传感器在人血清基质中具有优异的选择性和长期稳定性，证实了使用基于S - GQDs@Au - CNS的纳米材料作为有前景的传感探针用于实际应用以促进癌细胞超灵敏和高选择性检测的可行性。
