分子和计算方法在 3D 染色质结构中绘制调控元件。

Department of Biochemistry and Molecular Medicine and the Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90089, USA.

Epigenetics Chromatin. 2021 Mar 19;14(1):14. doi: 10.1186/s13072-021-00390-y.

Epigenetic marks do not change the sequence of DNA but affect gene expression in a cell-type specific manner by altering the activities of regulatory elements. Development of new molecular biology assays, sequencing technologies, and computational approaches enables us to profile the human epigenome in three-dimensional structure genome-wide. Here we describe various molecular biology techniques and bioinformatic tools that have been developed to measure the activities of regulatory elements and their chromatin interactions. Moreover, we list currently available three-dimensional epigenomic data sets that are generated in various human cell types and tissues to assist in the design and analysis of research projects.

表观遗传标记不会改变 DNA 的序列，但通过改变调控元件的活性，以细胞类型特异性的方式影响基因表达。新的分子生物学检测、测序技术和计算方法的发展使我们能够在全基因组三维结构上描绘人类表观基因组。在这里，我们描述了各种分子生物学技术和生物信息学工具，这些技术和工具已被开发用于测量调控元件及其染色质相互作用的活性。此外，我们列出了目前在各种人类细胞类型和组织中生成的三维表观基因组数据集，以协助研究项目的设计和分析。