Theranexus, Lyon, France; Lyon Neuroscience Research Center, Université de Lyon, CNRS, INSERM, Bron, France.
Theranexus, Lyon, France.
J Neurosci Methods. 2021 May 1;355:109139. doi: 10.1016/j.jneumeth.2021.109139. Epub 2021 Mar 16.
Recent advances using functional ultrasound (fUS) imaging have opened new avenues to evaluate brain activity through the regional monitoring of cerebral blood volume (CBV) dynamics. In particular, this technology paves the way for understanding physiological or pathological cerebral processes or exploring the pharmacological profiles of new drugs targeting brain disorders. One of the main difficulties of this technology is the lack of standardized and validated tools, in particular relevant brain atlases, to help improving the accuracy, automation and reproducibility of fUS data analysis.
Here, we demonstrate the possibility to use the MRI-validated SIGMA brain atlas in rat to perform fast and precise analysis of CBV changes in numerous functionally relevant regions of interest using fUS imaging. We applied this atlas to a dataset obtained in anesthetized rats evaluating the cerebral effects of atomoxetine, a norepinephrine reuptake inhibitor currently marketed in attention-deficit/hyperactivity-disorder.
This approach enabled to show the subregional effects of atomoxetine in the rat with very few inter-individual differences in some areas, such as the dentate gyrus.
We show the feasibility of inter-individual registration of 2D pharmaco-fUS data and subsequent detailed analysis using the SIGMA atlas.
最近利用功能超声（fUS）成像的进展为通过区域性监测脑血容量（CBV）动力学来评估大脑活动开辟了新途径。 特别是，这项技术为理解生理或病理大脑过程或探索针对大脑疾病的新药的药理学特征铺平了道路。 该技术的主要困难之一是缺乏标准化和经过验证的工具，特别是相关的大脑图谱，以帮助提高 fUS 数据分析的准确性、自动化和可重复性。
在这里，我们展示了使用经过 MRI 验证的 SIGMA 大脑图谱在大鼠中进行快速和精确分析功能相关感兴趣区域中 CBV 变化的可能性，使用 fUS 成像。 我们将该图谱应用于在麻醉大鼠中获得的数据集，以评估去甲肾上腺素再摄取抑制剂阿托西汀（目前在注意力缺陷/多动障碍中销售）对大脑的影响。
该方法能够显示阿托西汀在大鼠中的亚区域作用，在某些区域（如齿状回），个体间差异非常小。
我们展示了使用 SIGMA 图谱对 2D 药物 fUS 数据进行个体间配准和随后进行详细分析的可行性。
