Charité - Universitätsmedizin Berlin, Department of Rheumatology and Clinical Immunology, 10117, Berlin, Germany.
Immune Dynamics, Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute, Charitéplatz 1, 10117, Berlin, Germany.
Nat Commun. 2021 Mar 19;12(1):1737. doi: 10.1038/s41467-021-21994-8.
Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known as tissue-resident cells, their precise localization and interactions with the microenvironment are still unclear. Here we combine a multiplexed immunofluorescence technique and a customized computational, open-source analysis pipeline to unambiguously identify CD127 ILCs in situ and characterize these cells and their microenvironments. Moreover, we reveal the transcription factor IRF4 as a marker for tonsillar ILC3, and identify conserved stromal landmarks characteristic for ILC localization. We also show that CD127 ILCs share tissue niches with plasma cells in the tonsil. Our works thus provide a platform for multiparametric histological analysis of ILCs to improve our understanding of ILC biology.
先天淋巴细胞 (ILC) 在近几年作为免疫反应和生物学过程的重要调节因子而出现。尽管 ILC 主要被认为是组织驻留细胞，但它们的确切定位和与微环境的相互作用仍不清楚。在这里，我们结合了多重免疫荧光技术和定制的计算、开源分析管道，以明确鉴定原位 CD127 ILC，并对这些细胞及其微环境进行特征描述。此外，我们还揭示了转录因子 IRF4 作为扁桃体 ILC3 的标志物，并确定了 ILC 定位的保守基质标志。我们还表明，CD127 ILC 与扁桃体中的浆细胞共享组织小生境。因此，我们的工作为 ILC 的多参数组织学分析提供了一个平台，以提高我们对 ILC 生物学的理解。
