Guleken Zozan, Depciuch Joanna, Ege Hasan, İlbay Gül, Kalkandelen Cevriye, Ozbeyli Dilek, Bulut Huri, Sener Goksel, Tarhan Nevzat, Erdem Kuruca Serap
Department of Physiology, Uskudar University Faculty of Medicine, Istanbul, Turkey.
Institute of Nuclear Physics Polish Academy of Science, 31-342 Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jun 5;254:119639. doi: 10.1016/j.saa.2021.119639. Epub 2021 Mar 9.
Diabetic wounds have a slow healing process and easy to be infected. In addition to current drug treatments, supportive approaches are needed for diabetic wound treatment. In this study, we aimed to load Aloe Vera (AV) and Hypericum perforatum oil (HPO) with PCL/Ge (Poly (ɛ-caprolactone)/Gelatine) polymeric biodegradable by electrospinning method into nanofiber dressings on an experimental diabetic wound model to compare the diabetic wound healing effect. Changes in the amount and chemical structure of phospholipids, proteins, and lipids were investigated in the blood and serum samples of the animals using Fourier transform infrared (FTIR) analysis. To evaluate biological events associated with the wound repair process in inflammatory phase we used oxidant and antioxidant status to determine the healing status of wounds such as Total antioxidant status (TAS), Total oxidant level (TOS) and tumor necrosis factor alpha (TNF-α) levels. TOS level increased in DM groups and decreased in the AV and HPO group. Oxidative stress index decreased and TNF-α level increased in the HPO group. FTIR spectra showed changes in the phospholipids, proteins, and carbon chain of lipids in the whole blood as well as serum of DM rats. FTIR spectra combined with Principal component analysis (PCA) showed, that treated DM rats by AV and HPO caused return chemical structure of blood and serum to this observed in control group. Higher similarity with control group for HPO rats was observed. HPO is better than AV in the alternative for healing on diabetic wound. Thus, we have demonstrated that IR spectroscopy and multivariate data analysis and biochemical assays are consistent and correlative with each other.
糖尿病伤口愈合过程缓慢且易感染。除了现有的药物治疗外，糖尿病伤口治疗还需要支持性方法。在本研究中，我们旨在通过静电纺丝法将芦荟（AV）和贯叶连翘油（HPO）与聚己内酯/明胶（PCL/Ge）聚合物生物可降解材料负载到纳米纤维敷料中，用于实验性糖尿病伤口模型，以比较糖尿病伤口的愈合效果。使用傅里叶变换红外（FTIR）分析研究动物血液和血清样本中磷脂、蛋白质和脂质的含量及化学结构变化。为了评估炎症期与伤口修复过程相关的生物学事件，我们使用氧化和抗氧化状态来确定伤口的愈合状态，如总抗氧化状态（TAS）、总氧化水平（TOS）和肿瘤坏死因子α（TNF-α）水平。糖尿病组TOS水平升高，AV和HPO组TOS水平降低。HPO组氧化应激指数降低，TNF-α水平升高。FTIR光谱显示糖尿病大鼠全血和血清中磷脂、蛋白质和脂质碳链的变化。FTIR光谱结合主成分分析（PCA）表明，AV和HPO治疗的糖尿病大鼠使血液和血清的化学结构恢复到对照组观察到的状态。观察到HPO大鼠与对照组的相似度更高。在糖尿病伤口愈合方面，HPO比AV更好。因此，我们证明了红外光谱、多变量数据分析和生化检测相互一致且相关。
