Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Physiology, Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
J Hazard Mater. 2021 Aug 5;415:125633. doi: 10.1016/j.jhazmat.2021.125633. Epub 2021 Mar 12.
The immediate and the late effects of inhaled Paraquat (PQ) on systemic and lung inflammation and oxidative stress were investigated. Rats were exposed to saline (control group) and two doses of inhaled PQ (27 and 54 mg/m) and studied variables were measured: 1) one day after the end of PQ exposure as "immediate condition", 2) 16 days after the end of PQ exposure as "late condition". Total and differential white blood cells (WBC) counts, lipid peroxidation and nitrite were increased but thiol, superoxide dismutase and catalase in the blood and BALF as well as methacholine EC50 was reduced in both conditions in the animals exposed to PQ compared to control groups (p < 0. 05 to p < 0.001). Most studied parameters in the immediate condition were significantly higher than the late condition (p < 0.05 to p < 0.001). Systemic and lung inflammation and oxidative stress due to inhaled PQ in both the immediate and the late conditions were shown. Although most measured parameters in the immediate condition were higher, all variables were significantly different with the control group even in late condition, indicating a long-term effect of inhaled PQ toxicity, which may help in a more effective treatment of PQ poising in the future.
研究了吸入百草枯（PQ）对全身和肺部炎症及氧化应激的即刻和迟发效应。大鼠暴露于生理盐水（对照组）和两种剂量的吸入 PQ（27 和 54 mg/m），并测量了以下变量：1）PQ 暴露结束后一天作为“即刻条件”，2）PQ 暴露结束后 16 天作为“迟发条件”。与对照组相比，暴露于 PQ 的动物在两种情况下的全血和 BALF 中的总白细胞和白细胞分类计数、脂质过氧化和亚硝酸盐增加，但血液和 BALF 中的硫醇、超氧化物歧化酶和过氧化氢酶以及乙酰甲胆碱 EC50 降低（p<0.05 至 p<0.001）。即刻条件下大多数研究参数均显著高于迟发条件（p<0.05 至 p<0.001）。结果显示，吸入 PQ 会导致即刻和迟发情况下的全身和肺部炎症及氧化应激。尽管即刻条件下的大多数测量参数较高，但即使在迟发条件下，所有变量与对照组相比均有显著差异，这表明吸入 PQ 毒性具有长期效应，这可能有助于未来更有效地治疗 PQ 中毒。
