Vemuri R, Mersel M, Heller M, Pinson A
Laboratory for Myocardial Research, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Mol Cell Biochem. 1988 Jan;79(1):39-46. doi: 10.1007/BF00229396.
Cultured heart cells have been shown useful for investigating states of oxygen and volume restrictions, simulating anoxia and ischemia-like states at cellular levels. The sarcolemma has been implicated as one of the early sites of ischemic damage; therefore, lactoperoxidase catalyzed radioiodination was used to study accessibility of the sarcolemmal lipid moieties to this enzymatic probe, reflecting their exposure to the extracellular environment, hence the biophysical state of the sarcolemma. These studies have shown that within one hour of 'ischemic' injuries: (1) The degree of labelling in the total phospholipid fraction is considerably increased; and (2) Profound changes in the relative extent of labelling of different phospholipid classes were observed. The PE/PC labelling ratio increases dramatically with the progress of ischemia-like state. We suggest that early during ischemic injury, reorganization of the cell surface phospholipids occurs and discuss possible relations to the energy charge of the cell.
培养的心脏细胞已被证明可用于研究氧气和容量限制状态，在细胞水平模拟缺氧和缺血样状态。肌膜被认为是缺血损伤的早期部位之一；因此，利用乳过氧化物酶催化的放射性碘化来研究肌膜脂质部分对这种酶探针的可及性，反映它们与细胞外环境的接触情况，从而反映肌膜的生物物理状态。这些研究表明，在“缺血”损伤的一小时内：（1）总磷脂部分的标记程度显著增加；（2）观察到不同磷脂类别的标记相对程度发生了深刻变化。随着缺血样状态的进展，PE/PC标记率急剧增加。我们认为在缺血损伤早期，细胞表面磷脂会发生重组，并讨论了其与细胞能量状态的可能关系。
