Metchi Donfack Mireille Flaure, Atsamo Albert Donatien, Temdié Guemmogne Roméo Joël, Ngouateu Kenfack Omer Bébé, Dongmo Alain Bertrand, Dimo Théophile
Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundéré, Cameroon.
Evid Based Complement Alternat Med. 2021 Mar 5;2021:6668919. doi: 10.1155/2021/6668919. eCollection 2021.
stem-bark is used in Cameroonian traditional medicine to treat cardiovascular diseases including hypertension. In previous studies, the methanol/methylene chloride stem-bark extract of (MMVC) showed a preventive activity in L-NAME-induced hypertension and improved blood pressure of spontaneously hypertensive rats. The present study investigated the curative effects in L-NAME-induced hypertensive rats (LNHR). Hypertension was induced in rats by oral administration of L-NAME (40 mg/kg/day) for 28 days. The animals were divided into 2 groups: one group of 5 rats receiving distilled water (10 ml/kg) and another 20 rats receiving L-NAME. At the end of 4 weeks of administration of L-NAME, the animals were divided into 4 groups of 5 rats each: one group of hypertensive rats receiving distilled water, another one receiving captopril (25 mg/kg), and two groups of hypertensive rats receiving MMVC at doses of 200 and 400 mg/kg, respectively. Body weight, food, and water intake were measured weekly. At the end of the treatment, blood pressure and heart rate were recorded by invasive method. Whole heart, left ventricle, kidneys, and liver were weighed. The effects of plant extract on lipid profile and oxidative stress markers, as well as markers of hepatic and renal functions were assessed spectrophotometrically according to well described protocols. Results show that L-NAME significantly increases the mean arterial blood pressure (MABP), atherogenic index, lipid profile, and creatinine and transaminase activities of normotensive rats. MMVC significantly reduced the blood pressure in LNHR. Body weight, food and water intake, left ventricular hypertrophy, antioxidant level, renal and hepatic markers, and lipid profile were improved by the treatment with MMVC. The curative effect of MMVC on L-NAME-induced hypertension is probably related to its antihypertensive, hypolipidemic, and antioxidant properties. These results confirmed the use of for the treatment of hypertension in traditional medicine.
在喀麦隆传统医学中，茎皮被用于治疗包括高血压在内的心血管疾病。在先前的研究中，[植物名称未给出]的甲醇/二氯甲烷茎皮提取物（MMVC）在L-硝基精氨酸甲酯（L-NAME）诱导的高血压中显示出预防活性，并改善了自发性高血压大鼠的血压。本研究调查了MMVC对L-NAME诱导的高血压大鼠（LNHR）的治疗效果。通过口服L-NAME（40毫克/千克/天）连续28天诱导大鼠高血压。将动物分为2组：一组5只大鼠接受蒸馏水（10毫升/千克），另一组20只大鼠接受L-NAME。在给予L-NAME 4周结束时，将动物分为4组，每组5只大鼠：一组高血压大鼠接受蒸馏水，另一组接受卡托普利（25毫克/千克），两组高血压大鼠分别接受200和400毫克/千克剂量的MMVC。每周测量体重、食物和水摄入量。治疗结束时，通过侵入性方法记录血压和心率。称量全心、左心室、肾脏和肝脏的重量。根据详细描述的方案，通过分光光度法评估植物提取物对脂质谱和氧化应激标志物以及肝肾功能标志物的影响。结果表明，L-NAME显著升高了正常血压大鼠的平均动脉血压（MABP）、致动脉粥样硬化指数、脂质谱以及肌酐和转氨酶活性。MMVC显著降低了LNHR的血压。MMVC治疗改善了体重、食物和水摄入量、左心室肥厚、抗氧化水平、肾和肝标志物以及脂质谱。MMVC对L-NAME诱导的高血压的治疗作用可能与其降压、降血脂和抗氧化特性有关。这些结果证实了[植物名称未给出]在传统医学中用于治疗高血压的用途。
