Moreno Débora Antunes Neto, Saladini Mirela Santos, Viroel Fabia Judice Marques, Dini Murilo Melo Juste, Pickler Thaisa Borim, Amaral Filho Jorge, Dos Santos Carolina Alves, Hanai-Yoshida Valquíria Miwa, Grotto Denise, Gerenutti Marli, Hyslop Stephen, Oshima-Franco Yoko
Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.
Veterinary Medicine Graduate Course, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.
Adv Pharm Bull. 2021 Jan;11(1):130-136. doi: 10.34172/apb.2021.014. Epub 2020 Nov 7.
In this work, the potential usefulness of silver nanoparticles (AgNPs) for treating burn wounds was examined. Second-degree burns were induced in male Wistar rats by touching the skin with a heated (70°C) metallic device for 10 s, after which the animals were randomly allocated to one of two groups: control (n=8, treated with sterile saline) and experimental (n=8, treated with AgNPs, 0.081 mg/mL; 50 µL applied to the burn surface). Seven, 14, 21 and 28 days after lesion induction two rats from each group were killed and blood samples were collected for a complete blood count and to assess oxidative stress. The livers were examined macroscopically and skin samples were collected for histological analysis. Macroscopically, wound healing and skin remodeling in the experimental group were similar to the saline-treated rats. Likewise, there were no significant differences in the histological parameters between the two groups. However, treatment with AgNPs caused a persistent reduction in white blood cell (WBC) counts throughout the experiment, whereas platelet counts increased on days 7 and 28 but decreased on days 14 and 21; there was also an increase in the blood concentration of reduced glutathione on day 7 followed by a decrease on days 21 and 28. There were no significant changes in blood glutathione peroxidase (GSH-Px) and catalase (CAT) activities or in the serum concentration of thiobarbituric acid reactive substances. The findings of this study raise questions about the potential transitory effects of AgNPs based on the changes in WBC and platelet counts, blood glutathione concentrations and macroscopic hepatic alterations.
在本研究中，考察了银纳米颗粒（AgNPs）治疗烧伤创面的潜在效用。通过用加热至70°C的金属器械接触雄性Wistar大鼠皮肤10秒诱导二度烧伤，之后将动物随机分为两组：对照组（n = 8，用无菌生理盐水处理）和实验组（n = 8，用AgNPs处理，0.081 mg/mL；50 μL涂抹于烧伤表面）。损伤诱导后7天、14天、21天和28天，每组处死两只大鼠，采集血样进行全血细胞计数并评估氧化应激。对肝脏进行宏观检查，并采集皮肤样本进行组织学分析。宏观上，实验组的伤口愈合和皮肤重塑与生理盐水处理的大鼠相似。同样，两组之间的组织学参数没有显著差异。然而，在整个实验过程中，用AgNPs处理导致白细胞（WBC）计数持续降低，而血小板计数在第7天和第28天增加，但在第14天和第21天降低；第7天还原型谷胱甘肽的血浓度也增加，随后在第21天和第28天降低。血谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性以及硫代巴比妥酸反应性物质的血清浓度没有显著变化。基于白细胞和血小板计数、血谷胱甘肽浓度以及肝脏宏观改变，本研究结果引发了关于AgNPs潜在短暂效应的疑问。
